Maternal Exposure to D-galactose Reduces Ovarian Reserve in Female Rat Offspring Later in Life.

IF 2.1 Q3 ENDOCRINOLOGY & METABOLISM International Journal of Endocrinology and Metabolism Pub Date : 2022-05-30 eCollection Date: 2022-04-01 DOI:10.5812/ijem-123206
Marzieh Rostami Dovom, Mahsa Noroozzadeh, Nariman Mosaffa, Abbas Piryaei, Azita Zadeh-Vakili, Mohammad-Amin Aabdollahifar, Maryam Rahmati, Mahbanoo Farhadi-Azar, Fahimeh Ramezani Tehrani
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引用次数: 2

Abstract

Background: Embryonic life is critical for the formation of ovaries in mammals, and the intrauterine environment may affect ovarian reserve.

Objectives: The present study aimed to investigate the impact of prenatal D-galactose exposure on ovarian reserve in female rat offspring in their later lives.

Methods: Ten pregnant Wistar rats were randomly divided into two groups. In one group, rats were fed with 35% D-galactose-enriched food from the third day to the end of pregnancy, and in the other group, rats were fed with a standard diet throughout pregnancy. Female offspring (prenatally galactose-exposed rats and non-exposed control rats) were examined in terms of hormonal levels [anti-Mullerian hormones (AMH), follicle-stimulating hormone (FSH), and estradiol (E2)] and ovarian histology at 45 - 50, 105 - 110, and 180 - 185 days of their age.

Results: The number of primordial follicles significantly decreased time-dependently in prenatally galactose-exposed rats compared to controls (P-value = 0.002). In addition, decreases in AMH (3.25 vs. 7.5 ng/mL; P = 0.000) and E2 (7.9 vs. 19.5 pg/mL; P = 0.000) and increases in FSH (6.5 vs. 0.8 mIU/mL; P < 0.007) were observed in galactose-exposed rats compared to controls at 45 - 50 days of age.

Conclusions: Prenatal exposure to D-galactose negatively affects ovarian reserve in female rats in their later lives. However, further investigation is needed to confirm our findings and explore underlying mechanisms.

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母鼠暴露于d -半乳糖会降低母鼠后代日后卵巢储备功能。
背景:胚胎生命对哺乳动物卵巢的形成至关重要,而宫内环境可能影响卵巢储备。目的:探讨产前d -半乳糖暴露对雌性大鼠后代卵巢储备功能的影响。方法:10只Wistar妊娠大鼠随机分为两组。其中一组大鼠从妊娠第3天至妊娠结束时喂食富含35% d -半乳糖的食物,另一组大鼠在妊娠期间喂食标准饮食。雌性后代(产前暴露于半乳糖的大鼠和未暴露于乳糖的对照大鼠)在其45 - 50、105 - 110和180 - 185天龄时进行激素水平[抗苗勒管激素(AMH)、促卵泡激素(FSH)和雌二醇(E2)]和卵巢组织学检查。结果:与对照组相比,产前暴露于半乳糖的大鼠原始卵泡数量明显减少(p值= 0.002)。此外,AMH降低(3.25 vs. 7.5 ng/mL;P = 0.000)和E2 (7.9 vs. 19.5 pg/mL;P = 0.000)和促卵泡刺激素升高(6.5 vs 0.8 mIU/mL;P < 0.007),暴露于半乳糖的大鼠在45 - 50日龄时与对照相比。结论:产前暴露于d -半乳糖会对雌性大鼠日后的卵巢储备产生负面影响。然而,需要进一步的研究来证实我们的发现并探索潜在的机制。
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CiteScore
3.10
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4.80%
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期刊介绍: The aim of the International Journal of Endocrinology and Metabolism (IJEM) is to increase knowledge, stimulate research in the field of endocrinology, and promote better management of patients with endocrinological disorders. To achieve this goal, the journal publishes original research papers on human, animal and cell culture studies relevant to endocrinology.
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