Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway.

IF 2.7 3区生物学 Q4 CELL BIOLOGY BMC Molecular and Cell Biology Pub Date : 2022-07-25 DOI:10.1186/s12860-022-00435-4

Nesa Janamo Berenjabad, Vahid Nejati, Jafar Rezaie

{"title":"Angiogenic ability of human endothelial cells was decreased following senescence induction with hydrogen peroxide: possible role of vegfr-2/akt-1 signaling pathway.","authors":"Nesa Janamo Berenjabad, Vahid Nejati, Jafar Rezaie","doi":"10.1186/s12860-022-00435-4","DOIUrl":null,"url":null,"abstract":"Background: Many attempts are used to discover mechanisms driving impaired angiogenesis in age-related diseases. Angiogenesis is highly regulated by different signaling pathways. Here, we investigated the angiogenesis potential of human endothelial cells (ECs) upon exposure to hydrogen peroxide (H2O2), a cellular senescent factor.Results: Data showed that the wound healing rate of HUVECs decreased upon incubation with H2O2 (P < 0.05). LOX activity and NO production were decreased in H2O2 treated cells (P < 0.05). Expression of miR-126 and VEGFR-2 up-regulated, while expression of miR-373 and HSP-70 up = regulated in H2O2 -induced cells (P < 0.05). In addition, we found that protein levels of p-Akt-1, VCAM-1, MMP-9, and IL-6 decreased in treated cells (P < 0.05).Conclusions: Our data showed that H2O2 reduced the angiogenic response of HUVECs in vitro, which may be due to impairment of the VEGFR-2 signaling pathway.","PeriodicalId":9099,"journal":{"name":"BMC Molecular and Cell Biology","volume":" ","pages":"31"},"PeriodicalIF":2.7000,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9310472/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Molecular and Cell Biology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12860-022-00435-4","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Many attempts are used to discover mechanisms driving impaired angiogenesis in age-related diseases. Angiogenesis is highly regulated by different signaling pathways. Here, we investigated the angiogenesis potential of human endothelial cells (ECs) upon exposure to hydrogen peroxide (H₂O₂), a cellular senescent factor.

Results: Data showed that the wound healing rate of HUVECs decreased upon incubation with H₂O₂ (P < 0.05). LOX activity and NO production were decreased in H₂O₂ treated cells (P < 0.05). Expression of miR-126 and VEGFR-2 up-regulated, while expression of miR-373 and HSP-70 up = regulated in H₂O₂ -induced cells (P < 0.05). In addition, we found that protein levels of p-Akt-1, VCAM-1, MMP-9, and IL-6 decreased in treated cells (P < 0.05).

Conclusions: Our data showed that H₂O₂ reduced the angiogenic response of HUVECs in vitro, which may be due to impairment of the VEGFR-2 signaling pathway.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

过氧化氢诱导衰老后，人内皮细胞的血管生成能力下降：可能与vegfr-2/akt-1信号通路有关。

背景：许多研究试图发现衰老相关疾病中血管生成受损的机制。血管生成受不同信号通路的高度调控。在这里，我们研究了暴露于过氧化氢（H2O2）（一种细胞衰老因子）后人内皮细胞（ECs）的血管生成潜力。结果：数据显示H2O2 （p2o2）处理细胞（p2o2）诱导的HUVECs伤口愈合速率降低。结论：我们的数据显示H2O2降低了HUVECs体外血管生成反应，这可能与VEGFR-2信号通路受损有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊