Zinc oxide nanoparticles and spironolactone-enhanced Nrf2/HO-1 pathway and inhibited Wnt/β-catenin pathway in adenine-induced nephrotoxicity in rats.

IF 5.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Redox Report Pub Date : 2022-12-01 DOI:10.1080/13510002.2022.2139947

Amira Awadalla, Eman T Hamam, Fardous F El-Senduny, Nisreen Mansour Omar, Mohamed R Mahdi, Nashwa Barakat, Omar A Ammar, Abdelaziz M Hussein, Ahmed A Shokeir, Salma M Khirallah

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引用次数: 3

Abstract

Objective: To investigate the renoprotective, the antioxidant, and the anti-inflammatory impact of a combination of SPL and ZnO-NPs to combat against chronic kidney disease (CKD).

Methods: In total, 50 males of rats were distributed into 5 groups (10 rats each); normal group, adenine sulfate (0.25% in diet for 10 days) (CKD) group. After the last dose of adenine sulfate, rats were divided into three groups: SPL + Adenine sulfate group; rats were treated orally by mixing SPL (20 mg/kg/day) into chow for 8 weeks, ZnO-NPs + Adenine sulfate group; rats were injected intraperitoneally with ZnO-NPs (5 mg/kg) three times weekly for 8 weeks, ZnO-NPs + SPL + Adenine sulfate group; rats were injected with the same previous doses for 8 weeks.

Results: Each of SPL and ZnO-NPs up-regulated antioxidant genes (Nrf2 and HO-1), down-regulated fibrotic and inflammatory genes (TGF-β1, Wnt7a, β-catenin, fibronectin, collagen IV, α-SMA, TNF-α, and IL-6) compared to CKD. Furthermore, a combination of SPL and ZnO-NPs resulted in a greater improvement in the measured parameters than a single treatment.

Conclusion: The therapeutic role of SPL was enhanced by the antioxidant and the anti-inflammatory role of ZnO-NPs, which presented a great renoprotective effect against CKD.

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氧化锌纳米颗粒和螺内酯在腺嘌呤诱导的大鼠肾毒性中增强Nrf2/HO-1通路，抑制Wnt/β-catenin通路。

目的:探讨SPL联合no - nps对慢性肾脏疾病(CKD)的保护、抗氧化和抗炎作用。方法:雄性大鼠50只，随机分为5组，每组10只;正常组、硫酸腺嘌呤(0.25%)组(CKD)。末次给药后，将大鼠分为三组:SPL +硫酸腺嘌呤组;将SPL (20 mg/kg/d)加入饲料中口服治疗8周，为ZnO-NPs +硫酸腺嘌呤组;大鼠腹腔注射ZnO-NPs (5 mg/kg)，每周3次，连续8周，ZnO-NPs + SPL +硫酸腺嘌呤组;大鼠注射相同剂量，持续8周。结果:与CKD相比，SPL和ZnO-NPs均上调抗氧化基因Nrf2和HO-1，下调纤维化和炎症基因TGF-β1、Wnt7a、β-catenin、纤维连接蛋白、胶原IV、α-SMA、TNF-α和IL-6。此外，与单一处理相比，SPL和ZnO-NPs的组合处理对测量参数的改善更大。结论:ZnO-NPs的抗氧化和抗炎作用增强了SPL的治疗作用，对CKD具有良好的肾保护作用。

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来源期刊

Redox Report 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.