BRAIN CHOLESTEROL. 8. EFFECT OF METHYLPHENIDATE (RITALIN) ON THE INCORPORATION OF SPECIFICALLY LABELED ACETATE.

Proceedings of the Society for Experimental Biology and Medicine Pub Date : 1965-04-01 DOI:10.3181/00379727-118-30003

J J KABARA

{"title":"BRAIN CHOLESTEROL. 8. EFFECT OF METHYLPHENIDATE (RITALIN) ON THE INCORPORATION OF SPECIFICALLY LABELED ACETATE.","authors":"J J KABARA","doi":"10.3181/00379727-118-30003","DOIUrl":null,"url":null,"abstract":"In order further to understand factors affecting cholesterol metabolism in adult animals, we have studied the effect of a central nervous system stimulant, methylphenidate (Ritalin), on brain cholesterol metabolism. Structurally, this compound is related to compounds having a hypocholesterolemic effect (1,2,3), and also acts as a central nervous system stimulant(4). A preliminary report of these experiments has been published (5). The present paper is a more complete study of the effect of this drug on acetate incorporation into brain cholesterol.Experimental. Radioactive precursors. Acetate-2-3H (10 mC/m-mole); acetate-l-14C (2.8 mC/m-mole); acetate-2-14C (1.5 mC/m-mole) were used simultaneously in these studies. Radioactive nutrients were dissolved in physiological saline, with benzyl alcohol (0.9%) added as a preservative. The resultant solutions were injected intraperitoneally.The drug (methylphenidate) Ritalin, donated by Ciba Pharmaceutical Co. (H. Sheppard), was prepared as a 1% solution in saline....","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"905-8"},"PeriodicalIF":0.0000,"publicationDate":"1965-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-118-30003","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Society for Experimental Biology and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3181/00379727-118-30003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 3

Abstract

In order further to understand factors affecting cholesterol metabolism in adult animals, we have studied the effect of a central nervous system stimulant, methylphenidate (Ritalin), on brain cholesterol metabolism. Structurally, this compound is related to compounds having a hypocholesterolemic effect (1,2,3), and also acts as a central nervous system stimulant(4). A preliminary report of these experiments has been published (5). The present paper is a more complete study of the effect of this drug on acetate incorporation into brain cholesterol.Experimental. Radioactive precursors. Acetate-2-3H (10 mC/m-mole); acetate-l-14C (2.8 mC/m-mole); acetate-2-14C (1.5 mC/m-mole) were used simultaneously in these studies. Radioactive nutrients were dissolved in physiological saline, with benzyl alcohol (0.9%) added as a preservative. The resultant solutions were injected intraperitoneally.The drug (methylphenidate) Ritalin, donated by Ciba Pharmaceutical Co. (H. Sheppard), was prepared as a 1% solution in saline....

查看原文