FACTORS INFLUENCING UPTAKE OF TRIIODOTHYRONINE-I-131 BY BULL SPERMATOZOA.

Abstract

Summary The uptake of triiodothyronine-I-131 by washed bull spermatozoa was found to have a positive association with sperm cell concentration and period of incubation with the labeled T3. Spermatozoa suspended in a simple salt solution (2.9% sodium citrate) had a higher uptake of T3 131 than spermatozoa diluted in a more complex ionic medium (N-J solution). The T3-I131 uptake by the sperm cells suspended in the 2 diluents increased at different rates with an increase in cell concentration. Within the range studied, addition of glucose to the 2 diluents had no effect on uptake of T3-I131 by spermatozoa. Sperm cell viability greatly influenced their uptake of T3-I131. Cells killed by continuous incubation at 38 °C or by treatment with acetic acid or sodium hydroxide had similar isotope uptake values which were significantly less than that of live cells. Spermatozoa killed by cold shock treatment, however, had the lowest uptake of T3-I131. Whereas, formaldehyde treatment of spermatozoa resulted in uptake values significantly greater than that of live cells. A positive association between uptake of T3-I131 and motility occurred with spermatozoa stored up to 18 days in different diluents and temperatures.