BIOLOGICAL PROPERTIES OF GUINEA PIG ANTI-INSULIN ANTIBODIES.

Abstract

Summary 1. Precipitating antibodies to insulin have been demonstrated in the guinea pig. 2. Two types of 7S anti-insulin antibodies were separated by zone electrophoresis and were shown to have different biological activities. The slower moving gamma 2 antibodies provoked hemagglutination and hemolysis in presence of complement. The faster moving gamma 1 antibodies gave PCA reactions in the guinea pig. 3. Oxidized B chain of insulin did not share antigenic determinants with the intact insulin molecule.