Determination of Fe(II) and Zn(II) by spectrophotometry, atomic absorption spectrometry and ions chromatography methods in VitrumR

Abstract

Metrian [3-mercapto-5-(2′-hydroxynaphtylazo-1′)-1,2,4-triazole]; Metriap [3-mercapto-5-(3′,4′-dihydroxyphenylazo-1′)-1,2,4-triazole]; Metriarez-γ [3-mercapto-5-(2′,4′-dihydroxy-3′-caroboxyphenylazo-1′)-1,2,4-triazole] and Metidarez-β [2-mercapto-5-(2′,4′-dihydroxy-5′-carboxyphenylazo-1′)-1,3,4-thiadiazole]-reagents were synthesized on Faculty of Pharmacy, Medical University of Lublin and used for the determination by different analytical methods of milligram quantities of Fe(II) and Zn(II) occurring together in pharmaceutical preparations, both multivitamin preparations and those containing microelements. The determination results of classical spectrophotometry (D₀), spectrophotometry of derivatives (D₁ and D₂), atomic absorption spectrometric (AAS) and ions chromatography (IC) were analyzed statistically and compared with declared amount. The advantages of the proposed method of Fe(II) and Zn(II) determination include it's excellent precision and reproducibility of results.