Henrik E Mei, Taketoshi Yoshida, Gwendolin Muehlinghaus, Falk Hiepe, Thomas Dörner, Andreas Radbruch, Bimba F Hoyer
{"title":"Phenotypic analysis of B-cells and plasma cells.","authors":"Henrik E Mei, Taketoshi Yoshida, Gwendolin Muehlinghaus, Falk Hiepe, Thomas Dörner, Andreas Radbruch, Bimba F Hoyer","doi":"10.1007/978-1-59745-402-5_1","DOIUrl":null,"url":null,"abstract":"<p><p>B-cells and antibody-secreting plasma cells are key players in protective immunity, but also in autoimmune disease. To understand their various functions in the initiation and maintenance of autoimmune pathology, a detailed dissection of their functional diversity is mandatory. This requires a detailed phenotypic classification of the diversity of B-cells. Here, technologies of immunocytometry and ELISpot are described in detail, and their value for phenotypic characterization of cells of the B lineage, as well as for preparative cell sorting, to further characterize them functionally and on the molecular level are described.</p>","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"136 ","pages":"3-18"},"PeriodicalIF":0.0000,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-402-5_1","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/978-1-59745-402-5_1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
Abstract
B-cells and antibody-secreting plasma cells are key players in protective immunity, but also in autoimmune disease. To understand their various functions in the initiation and maintenance of autoimmune pathology, a detailed dissection of their functional diversity is mandatory. This requires a detailed phenotypic classification of the diversity of B-cells. Here, technologies of immunocytometry and ELISpot are described in detail, and their value for phenotypic characterization of cells of the B lineage, as well as for preparative cell sorting, to further characterize them functionally and on the molecular level are described.
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