Increased expression levels of DLX5 inhibit the development of the nervous system

IF 1.7 4区医学 Q3 DEVELOPMENTAL BIOLOGY International Journal of Developmental Neuroscience Pub Date : 2023-09-28 DOI:10.1002/jdn.10300

Tingting Liao, Xia Xu, Junzi Wu, Yi Xie, Jianying Yan

{"title":"Increased expression levels of DLX5 inhibit the development of the nervous system","authors":"Tingting Liao, Xia Xu, Junzi Wu, Yi Xie, Jianying Yan","doi":"10.1002/jdn.10300","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>Preeclampsia is a hypertensive disorder of pregnancy. DLX5 plays an important role in the migration and differentiation of subglobus pallidus precursor cells.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>We established a zebrafish line expressing high levels of DLX5 and investigated changes in behavior and development of the nervous system.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The ratios of brain volume area to whole body area at 96 hpf zebrafish in the experimental group (gRNA + CasRx) were significantly lower than the WT group and the negative control group (casRx) (<i>P</i> < 0.01). Behavioral trajectory distances and movement speeds exhibited by the 6th day of development in zebrafish in the experimental group (gRNA + CasRx) were significantly shorter (<i>P</i> < 0.01) and lower (<i>P</i> < 0.05) than the negative control group (gRNA + CasRx), respectively.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Data suggested that the increased expression levels of DLX5 can inhibit brain volume development and behavioral activities in zebrafish. Maybe the high expression levels of DLX5 in the pathological state of preeclampsia can inhibit the development of the nervous system in offspring.</p>\n </section>\n </div>","PeriodicalId":13914,"journal":{"name":"International Journal of Developmental Neuroscience","volume":"83 8","pages":"728-739"},"PeriodicalIF":1.7000,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jdn.10300","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Developmental Neuroscience","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jdn.10300","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

Preeclampsia is a hypertensive disorder of pregnancy. DLX5 plays an important role in the migration and differentiation of subglobus pallidus precursor cells.

Methods

We established a zebrafish line expressing high levels of DLX5 and investigated changes in behavior and development of the nervous system.

Results

The ratios of brain volume area to whole body area at 96 hpf zebrafish in the experimental group (gRNA + CasRx) were significantly lower than the WT group and the negative control group (casRx) (P < 0.01). Behavioral trajectory distances and movement speeds exhibited by the 6th day of development in zebrafish in the experimental group (gRNA + CasRx) were significantly shorter (P < 0.01) and lower (P < 0.05) than the negative control group (gRNA + CasRx), respectively.

Conclusions

Data suggested that the increased expression levels of DLX5 can inhibit brain volume development and behavioral activities in zebrafish. Maybe the high expression levels of DLX5 in the pathological state of preeclampsia can inhibit the development of the nervous system in offspring.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

DLX5表达水平的增加抑制了神经系统的发育。

前言：子痫前期是妊娠期的一种高血压疾病。DLX5在苍白球下前体细胞的迁移和分化中起着重要作用。方法：我们建立了一个表达高水平DLX5的斑马鱼系，并研究了行为和神经系统发育的变化。结果：大脑体积面积与全身面积之比为96 实验组中的hpf斑马鱼（gRNA + CasRx）显著低于WT组和阴性对照组（CasRx）（P 结论：数据表明，DLX5表达水平的增加可以抑制斑马鱼的脑容量发育和行为活动。DLX5在先兆子痫病理状态下的高表达水平可能会抑制后代神经系统的发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

International Journal of Developmental Neuroscience 医学-发育生物学

CiteScore

3.30

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： International Journal of Developmental Neuroscience publishes original research articles and critical review papers on all fundamental and clinical aspects of nervous system development, renewal and regeneration, as well as on the effects of genetic and environmental perturbations of brain development and homeostasis leading to neurodevelopmental disorders and neurological conditions. Studies describing the involvement of stem cells in nervous system maintenance and disease (including brain tumours), stem cell-based approaches for the investigation of neurodegenerative diseases, roles of neuroinflammation in development and disease, and neuroevolution are also encouraged. Investigations using molecular, cellular, physiological, genetic and epigenetic approaches in model systems ranging from simple invertebrates to human iPSC-based 2D and 3D models are encouraged, as are studies using experimental models that provide behavioural or evolutionary insights. The journal also publishes Special Issues dealing with topics at the cutting edge of research edited by Guest Editors appointed by the Editor in Chief. A major aim of the journal is to facilitate the transfer of fundamental studies of nervous system development, maintenance, and disease to clinical applications. The journal thus intends to disseminate valuable information for both biologists and physicians. International Journal of Developmental Neuroscience is owned and supported by The International Society for Developmental Neuroscience (ISDN), an organization of scientists interested in advancing developmental neuroscience research in the broadest sense.