Serum IgA augments adhesiveness of cultured lung microvascular endothelial cells and suppresses angiogenesis

IF 3.7 4区 医学 Q2 CELL BIOLOGY Cellular immunology Pub Date : 2023-09-16 DOI:10.1016/j.cellimm.2023.104769
Kazufumi Takada , Maho Suzukawa , Sayaka Igarashi , Yuuki Uehara , Shizuka Watanabe , Sahoko Imoto , Masaki Ishii , Yoshiteru Morio , Hirotoshi Matsui , Masahiro Akishita , Ken Ohta
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Abstract

Immunoglobulin A (IgA) is important in local immunity and is also abundant in the blood. This study aimed to evaluate the effects of serum IgA on cultured lung microvascular endothelial cells (HMVEC-Ls), which are involved in the pathogenesis of inflammatory lung diseases. Serum IgA induced adhesion molecules and inflammatory cytokine production from HMVEC-Ls, and enhanced adhesion of peripheral blood mononuclear cells to HMVEC-Ls. In contrast, migration, proliferation, and tube formation of HMVEC-Ls were significantly suppressed by serum IgA. Experiments with siRNAs and western blotting revealed that two known IgA receptors, β1,4-galactosyltransferase 1 (b4GALT1) and asialoglycoprotein receptor 1 (ASGR1), and mitogen-activated protein kinase and nuclear factor-kappa B pathways were partly involved in serum IgA-induced cytokine production by HMVEC-Ls. Collectively, serum IgA enhanced cytokine production and adhesiveness of HMVEC-L, with b4GALT1 and ASGR1 partially being involved, and suppressed angiogenesis. Thus, serum IgA may be targeted to treat inflammatory lung diseases.

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血清IgA增强培养的肺微血管内皮细胞的粘附性并抑制血管生成。
免疫球蛋白A(IgA)在局部免疫中很重要,在血液中也很丰富。本研究旨在评估血清IgA对培养的肺微血管内皮细胞(HMVEC Ls)的影响,这些细胞参与了炎症性肺部疾病的发病机制。血清IgA诱导HMVEC Ls产生粘附分子和炎性细胞因子,并增强外周血单核细胞与HMVEC L的粘附。相反,血清IgA显著抑制HMVEC Ls的迁移、增殖和管形成。siRNA和蛋白质印迹实验表明,两种已知的IgA受体,β1,4-半乳糖基转移酶1(b4GALT1)和去唾液酸糖蛋白受体1(ASGR1),以及促分裂原活化蛋白激酶和核因子κB途径,部分参与了HMVEC Ls产生血清IgA诱导的细胞因子。总的来说,血清IgA增强了细胞因子的产生和HMVEC-L的粘附性,b4GALT1和ASGR1部分参与其中,并抑制了血管生成。因此,血清IgA可以靶向治疗炎症性肺部疾病。
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来源期刊
Cellular immunology
Cellular immunology 生物-免疫学
CiteScore
8.20
自引率
2.30%
发文量
102
审稿时长
30 days
期刊介绍: Cellular Immunology publishes original investigations concerned with the immunological activities of cells in experimental or clinical situations. The scope of the journal encompasses the broad area of in vitro and in vivo studies of cellular immune responses. Purely clinical descriptive studies are not considered. Research Areas include: • Antigen receptor sites • Autoimmunity • Delayed-type hypersensitivity or cellular immunity • Immunologic deficiency states and their reconstitution • Immunologic surveillance and tumor immunity • Immunomodulation • Immunotherapy • Lymphokines and cytokines • Nonantibody immunity • Parasite immunology • Resistance to intracellular microbial and viral infection • Thymus and lymphocyte immunobiology • Transplantation immunology • Tumor immunity.
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