Orthodenticle Homeobox OTX1 Promotes Papillary Thyroid Carcinoma Progression and Is a Potential Prognostic Biomarker.

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY Genetics research Pub Date : 2023-09-21 eCollection Date: 2023-01-01 DOI:10.1155/2023/5513812

Jing Wei, Xin Wang, Kai Jiao

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Abstract

Papillary thyroid carcinoma (PTC) is the most common type of thyroid neoplasms, characterized by evidence of follicular cell differentiation. Orthodenticle homeobox 1 (OTX1) is a transcription factor which has been implicated in numerous diseases, including malignancies. The objective of this research was to explore the function of OTX1 in PTC. Immunohistochemistry (IHC) was employed to determine the protein level of OTX1 in PTC specimens. Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, a xenograft model on nude mice was established to investigate in vivo effects of OTX1. Our results revealed that OTX1 was significantly upregulated within specific PTC tissues and was remarkably correlated with unfavorable clinical outcomes in PTC. Silencing OTX1 resulted in a significant inhibition in cell viability and suppressed cell proliferation. In addition, in vivo experiments demonstrated that OTX1 silencing resulted in a significant suppression of tumor growth in nude mice. Collectively, these results suggest that OTX1 may play crucial roles in promoting PTC progression.

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口腔同源盒OTX1促进甲状腺乳头状癌的进展，是一种潜在的预后生物标志物。

乳头状甲状腺癌（PTC）是最常见的甲状腺肿瘤类型，其特征是滤泡细胞分化。口腔同源盒1（OTX1）是一种转录因子，与包括恶性肿瘤在内的多种疾病有关。本研究旨在探讨OTX1在PTC中的作用。采用免疫组化法（IHC）测定PTC组织中OTX1的蛋白水平。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑（MTT）测定法评估细胞活力。此外，建立了裸鼠异种移植物模型来研究OTX1的体内作用。我们的结果显示，OTX1在特定PTC组织中显著上调，并且与PTC的不良临床结果显著相关。沉默OTX1导致细胞活力的显著抑制和细胞增殖的抑制。此外，体内实验表明，OTX1沉默导致裸鼠肿瘤生长的显著抑制。总之，这些结果表明OTX1可能在促进PTC进展中发挥关键作用。

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.