DNA damage in foundry workers using non-invasive micronucleus cytome assay

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-10-01 DOI:10.1016/j.mrgentox.2023.503686

Hakimeh Nazari Khuniqi , Yahya Rasoulzadeh , Yousef Mohammadian

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Abstract

Workers in the foundry industry are exposed to hazardous chemical agents such as metal fumes, gases, vapor of molten metal, and respirable dust and hazardous physical agents such as heat, noise, and electromagnetic fields. Co-exposures to hazardous physical and chemical agents in foundry workplaces may cause DNA damage in workers. This study aimed to evaluate DNA damage in foundry workers. Thirty-three exposed foundry workers as a exposure groups and 33 non-exposed individuals as a control groups participated in this study. Buccal micronucleus cytome (BMCyt assay) assay was used to assess DNA damage. Results showed that foundry workers were under exposure to hazardous chemical and physical agents such as metal fumes and noise. The percentage of micronucleus (MN) cells in exposure group (0.59 ± 0.93 %) were statistically higher than control group (0.23 ± 0.23 %) (P < 0.05) %). Also, the percentage of nuclear bud cells and binucleated cells in exposure group were statistically higher than control group (P < 0.05). The percentage of differentiated normal cells were significantly higher in the control group compared to the exposed group (P < 0.05). Foundry workers are at risk of DNA damage; therefore, prevention measures need to be implemented to reduce exposure to air pollutants in foundry workplaces.

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使用非侵入性微核细胞仪测定铸造工人的DNA损伤。

铸造行业的工人会接触到危险的化学制剂，如金属烟雾、气体、熔融金属蒸汽、可吸入的灰尘和危险的物理制剂，如热、噪音和电磁场。在铸造厂工作场所共同接触危险的物理和化学制剂可能会导致工人的DNA损伤。本研究旨在评估铸造工人的DNA损伤。33名暴露的铸造工人作为暴露组，33名未暴露的个体作为对照组参与了本研究。用口腔微核细胞仪（BMCyt法）检测DNA损伤。结果表明，铸造工人暴露在危险的化学和物理因素中，如金属烟雾和噪音。微核率暴露组（0.59±0.93%）明显高于对照组（0.23±0.23%）（P

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.