Impact of prepubertal obesity induced by high-fat diet during lactation and post-weaning on puberty initiation and neuroendocrine function in a female mouse model.

Neuro endocrinology letters Pub Date : 2023-06-14
Mengmeng Shi, Shi Ren, Yue Zhang, Qinling Yang, Lingling Zhai
{"title":"Impact of prepubertal obesity induced by high-fat diet during lactation and post-weaning on puberty initiation and neuroendocrine function in a female mouse model.","authors":"Mengmeng Shi,&nbsp;Shi Ren,&nbsp;Yue Zhang,&nbsp;Qinling Yang,&nbsp;Lingling Zhai","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To explore the effects of prepubertal obesity induced by high-fat diet during lactation and post-weaning on puberty onset and the neuroendocrine changes before puberty onset in a female mouse model, which may explain obesity in children starting early puberty.</p><p><strong>Methods: </strong>A total of 72 female mice were assigned to the high fat diet group (HFD) and the control diet group (CONT) during lactation and post-weaning. The bodily indexes; pathological changes; and protein and gene expression levels in the hypothalamus were examined on postnatal days (P) 15, 28, and 45, respectively.</p><p><strong>Results: </strong>The average vaginal opening time in HFD mice occurred significantly earlier than that in CONT mice (p < 0.05). On P15, no significant difference in the MKRN3, kisspeptin, GPR54 and GnRH level between HFD and CONT mice was noted (p > 0.05). Whereas on P28 and 45, compared to CONT mice, GnRH expression in HFD mice was significantly increased (p < 0.05); kisspeptin and GPR54 expression in HFD mice was also significantly increased (p < 0.05); but the MKRN3 level in HFD mice was significantly lower than that in CONT mice (p < 0.05). On P15, 28, and 45, compared with CONT mice, miR-30b expression in HFD mice increased (p < 0.05). Compared to P15, miR-30b, KiSS-1, GPR54 and GnRH mRNA level increased significantly, however MKRN3 decreased significantly in HFD mice on P28 and 45 (p < 0.01).</p><p><strong>Conclusions: </strong>Prepubertal obesity induced by high-fat diet during lactation and post-weaning may advance the time of pubertal initiation in female mice. The increased expression of miR-30b, kisspeptin, GPR54 and GnRH, decreased the expression of MKRN3 may explain the early onset of puberty in obese female mice.</p>","PeriodicalId":94154,"journal":{"name":"Neuro endocrinology letters","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuro endocrinology letters","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Purpose: To explore the effects of prepubertal obesity induced by high-fat diet during lactation and post-weaning on puberty onset and the neuroendocrine changes before puberty onset in a female mouse model, which may explain obesity in children starting early puberty.

Methods: A total of 72 female mice were assigned to the high fat diet group (HFD) and the control diet group (CONT) during lactation and post-weaning. The bodily indexes; pathological changes; and protein and gene expression levels in the hypothalamus were examined on postnatal days (P) 15, 28, and 45, respectively.

Results: The average vaginal opening time in HFD mice occurred significantly earlier than that in CONT mice (p < 0.05). On P15, no significant difference in the MKRN3, kisspeptin, GPR54 and GnRH level between HFD and CONT mice was noted (p > 0.05). Whereas on P28 and 45, compared to CONT mice, GnRH expression in HFD mice was significantly increased (p < 0.05); kisspeptin and GPR54 expression in HFD mice was also significantly increased (p < 0.05); but the MKRN3 level in HFD mice was significantly lower than that in CONT mice (p < 0.05). On P15, 28, and 45, compared with CONT mice, miR-30b expression in HFD mice increased (p < 0.05). Compared to P15, miR-30b, KiSS-1, GPR54 and GnRH mRNA level increased significantly, however MKRN3 decreased significantly in HFD mice on P28 and 45 (p < 0.01).

Conclusions: Prepubertal obesity induced by high-fat diet during lactation and post-weaning may advance the time of pubertal initiation in female mice. The increased expression of miR-30b, kisspeptin, GPR54 and GnRH, decreased the expression of MKRN3 may explain the early onset of puberty in obese female mice.

分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
哺乳期和断奶后高脂肪饮食诱导的青春期前肥胖对雌性小鼠模型青春期发育和神经内分泌功能的影响。
目的:在雌性小鼠模型中,探讨哺乳期和断奶后高脂肪饮食诱导的青春期前肥胖对青春期开始的影响以及青春期开始前的神经内分泌变化,这可能解释青春期早期儿童的肥胖。方法:将72只雌性小鼠分为哺乳期和断奶后的高脂饮食组(HFD)和对照饮食组(CONT)。身体指标;病理变化;分别在出生后第15、28和45天检测下丘脑中的蛋白质和基因表达水平(P)。结果:HFD小鼠平均阴道开放时间明显早于CONT小鼠(p<0.05)。在P15,HFD和CONT小鼠的MKRN3、kisspeptin、GPR54和GnRH水平没有显著差异(p>0.05)。而在P28和45,与CONT小鼠相比,HFD小鼠的GnRH表达显著增加(p<0.05);kisspeptin和GPR54在HFD小鼠中的表达也显著增加(p<0.05);但HFD小鼠的MKRN3水平明显低于CONT小鼠(p结论:哺乳期和断奶后高脂饮食诱导的青春期前肥胖可能提前了雌性小鼠青春期发育的时间。miR-30b、kisspeptin、GPR54和GnRH的表达增加,MKRN3的表达减少,这可能解释了肥胖雌性小鼠青春期提前的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Building skills in cognitive behavioral supervision. Hybrid Artificial Intelligence Solution Combining Convolutional Neural Network and Analytical Approach Showed Higher Accuracy in A-lines Detection on Lung Ultrasound in Thoracic Surgery Patients Compared with Radiology Resident. Impact of an over-the-counter "sleep lotion" on human salivary melatonin levels and sleep quality: a randomized controlled trial. New approach and strategy in the assessment of labour pain - Czech version of the labour coping pain assessment tool. Protective effects and mechanisms of deer blood phospholipids (DBP) on zearalenone-induced oxidative damage in swine Sertoli cells.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1