Comparison of SYBR green I and lactate dehydrogenase antimalarial in vitro assay in Plasmodium falciparum field isolates

IF 1.3 4区 医学 Q4 PHARMACOLOGY & PHARMACY Journal of pharmacological and toxicological methods Pub Date : 2023-09-29 DOI:10.1016/j.vascn.2023.107472
Joseph Hawadak , Shewta Chaudhry , Veena Pande , Vineeta Singh
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Abstract

Several assay methods are in use for monitoring the drug sensitivity of malaria parasites and screening new antimalarial drugs. Plasmodium lactate dehydrogenase (pLDH) and SYBR Green I in vitro assays were used to evaluate the drug efficacy of Chloroquine, Artemisinin and Azadirachta indica silver nano particles against Plasmodium falciparum 3D7 strain. The half-maximal inhibitory concentration (IC50) of each compound was estimated with non-linear regression model – dose-response analysis. The consistency between two methods was analysed with Cohen's kappa coefficient, interclass correlation and Bland-Altman plots. No statistical difference was found between IC50 values determined by both assays (p = 0.714). The proportion of resistant isolates to chloroquine according to SYBR green I (43.48%) and pLDH (34.78%) assays were similar (z = 0.302; p = 0.762) with significant concordant between methods (k = 0.819, p < 0.001). The results of pLDH Qualisa assay was comparable with classic SYBR green I assay and can be potentially useful in antimalarial drug efficacy surveillance.

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SYBR green I和乳酸脱氢酶在恶性疟原虫分离株中的体外抗疟试验比较。
几种检测方法正在用于监测疟原虫的药物敏感性和筛选新的抗疟药物。采用乳酸疟原虫脱氢酶(pLDH)和SYBR Green I体外试验,评价了氯喹、青蒿素和印楝银纳米粒子对恶性疟原虫3D7株的药效。各化合物的半数最大抑制浓度(IC50)用非线性回归模型-剂量反应分析法估算。用Cohen’s kappa系数、类间相关性和Bland-Altman图分析了两种方法的一致性。两种测定的IC50值之间没有发现统计学差异(p = 0.714)。根据SYBR green I(43.48%)和pLDH(34.78%)测定,对氯喹的抗性分离株的比例相似(z = 0.302;p = 0.762),方法之间显著一致(k = 0.819,p
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来源期刊
Journal of pharmacological and toxicological methods
Journal of pharmacological and toxicological methods PHARMACOLOGY & PHARMACY-TOXICOLOGY
CiteScore
3.60
自引率
10.50%
发文量
56
审稿时长
26 days
期刊介绍: Journal of Pharmacological and Toxicological Methods publishes original articles on current methods of investigation used in pharmacology and toxicology. Pharmacology and toxicology are defined in the broadest sense, referring to actions of drugs and chemicals on all living systems. With its international editorial board and noted contributors, Journal of Pharmacological and Toxicological Methods is the leading journal devoted exclusively to experimental procedures used by pharmacologists and toxicologists.
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