Effects of gastrodin on the expression of brain aging-related genes in SAM/P-8 mice based on network pharmacology

Ibrain Pub Date : 2022-11-06 DOI:10.1002/ibra.12076

Nan Zhao, Rui Jiang, Jun-Jie Cheng, Qiu-Xia Xiao

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Abstract

Background

Gastrodin can reduce neuronal damage through multiple targets and pathways, and can be useful in preventing and treating degenerative lesions of the central nervous system, but the specific mechanism has not been elucidated.

Methods

The aging-related genes in the hippocampus and the frontal cortex were detected in adult and aged mice treated with gastrodin or not. In addition, we collected the target genes of gastrodin and aging from a network database, and a Venn diagram was created to obtain the intersection target genes of gastrodin and aging. Then, the String database was used to analyze the protein–protein interactions (PPIs) between aging-related genes and the target genes of gastrodin and aging. The “drug–disease–target–pathway” network was constructed using Cytoscape 3.7.2 software, and the main mechanism and pathway of key genes were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). Finally, the reliability of these key genes was further verified by molecular docking technology.

Results

The results showed that 6 out of 10 genes related to brain aging were differentially expressed after gastrodin intervention. Moreover, there were 11 key genes between gastrodin and differentially expressed genes related to brain aging. GO and KEGG results suggested that material metabolism and carbohydrate digestion and absorption were associated with the pathological mechanism of gastrodin antiaging. Molecular docking results also confirmed the good binding activity of gastrodin to the key genes.

Conclusion

Gastrodin plays a potential role in antiaging by regulating substance metabolism and carbohydrate digestion and absorption.

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基于网络药理学的天麻素对SAM/P-8小鼠脑衰老相关基因表达的影响。

背景：天麻素可以通过多种靶点和途径减少神经元损伤，并可用于预防和治疗中枢神经系统退行性病变，但其具体机制尚未阐明。方法：检测天麻素对成年和老龄小鼠海马和额叶皮层衰老相关基因的影响。此外，我们从网络数据库中收集了天麻素与衰老的靶基因，并创建了Venn图来获得天麻素与老化的交叉靶基因。然后，使用String数据库分析衰老相关基因与天麻素和衰老靶基因之间的蛋白质-蛋白质相互作用（PPIs）。使用Cytoscape 3.7.2软件构建“药物-疾病-靶向通路”网络，并通过京都基因与基因组百科全书（KEGG）和基因本体论（GO）分析关键基因的主要机制和通路。最后，通过分子对接技术进一步验证了这些关键基因的可靠性。结果：天麻素干预后，10个脑衰老相关基因中有6个差异表达。此外，天麻素与脑衰老相关的差异表达基因之间存在11个关键基因。GO和KEGG结果表明，物质代谢和碳水化合物的消化吸收与天麻素抗衰老的病理机制有关。分子对接结果也证实了天麻素与关键基因具有良好的结合活性。结论：天麻素通过调节物质代谢和碳水化合物的消化吸收，具有潜在的抗衰老作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Ibrain

CiteScore

0.90

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0.00%

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