Expression analysis of plvap in mouse heart development, homeostasis and injury

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY Gene Expression Patterns Pub Date : 2023-09-28 DOI:10.1016/j.gep.2023.119343

Yu Sui , Shan Kou , Kaixin Ge , Jinjun Cao , Chen Liu , Hui Zhang

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Abstract

Plasmalemma vesicle associated protein (PLVAP) is commonly considered to be specifically expressed in endothelial cells in which it localized to diaphragms of caveolae, fenestrae, and transendothelial channels. PLVAP is reported to be an important regulator of heart development and a novel target to promote cardiac repair in the ischemic heart. However, the dynamics of plvap expression in heart development, homeostasis and pathology have not been comprehensively described. In this study, we analyzed the temporal and spatial expression of plvap in mouse heart under different conditions. We found that, during embryonic and neonatal stages, PLVAP was detected in endocardial endothelial cells, epicardial mesothelial cells, and a small amount of coronary vascular endothelial cells. In adult heart, PLVAP was also identified in endocardial cells and a few coronary vascular endothelial cells. However, epicardial expression of PLVAP was lost during postnatal heart development and cannot be detected in mouse heart by immunostaining since 3-week-old. We also analyzed the expression of plvap in a model of cardiac hypertrophy and failure induced by transverse aortic constriction surgery, and identified expression of PLVAP in endocardial cells and coronary vascular endothelial cells in the injured heart. This study provides new evidence to better understand the role of plvap in mouse heart development and injury.

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plvap在小鼠心脏发育、稳态和损伤中的表达分析。

Plasmalemma囊泡相关蛋白（PLVAP）通常被认为在内皮细胞中特异性表达，其定位于小窝、开窗和跨内皮通道的隔膜。据报道，PLVAP是心脏发育的重要调节因子，也是促进缺血性心脏修复的新靶点。然而，plvap在心脏发育、稳态和病理中的表达动力学尚未得到全面描述。在本研究中，我们分析了不同条件下plvap在小鼠心脏中的时空表达。我们发现，在胚胎和新生儿阶段，在心内膜内皮细胞、心外膜间皮细胞和少量冠状血管内皮细胞中检测到PLVAP。在成人心脏中，在心内膜细胞和少数冠状血管内皮细胞中也发现了PLVAP。然而，PLVAP的心外膜表达在出生后心脏发育过程中丢失，并且从3周大起就无法通过免疫染色在小鼠心脏中检测到。我们还分析了plvap在主动脉横缩手术诱导的心脏肥大和衰竭模型中的表达，并鉴定了plvap在受损心脏的心内膜细胞和冠状血管内皮细胞中的表达。本研究为更好地了解plvap在小鼠心脏发育和损伤中的作用提供了新的证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation

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