Umbilical Cord-Derived Mesenchymal Stem Cells Improve TGF-β, α-SMA and Collagen on Erectile Dysfunction in Streptozotocin-Induced Diabetic Rats.

Medical archives (Sarajevo, Bosnia and Herzegovina) Pub Date : 2022-02-01 DOI:10.5455/medarh.2022.76.4-11

Ade Indra Mukti, Syafruddin Ilyas, Syah Mirsya Warli, Agung Putra, Nur Rasyid, Delfitri Munir, Kamal Basri Siregar, Muhammad Ichwan

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引用次数: 3

Abstract

Background: A Erectile dysfunction (ED) is one of the well-known comorbidities in males with diabetes mellitus (DM), whose pathogenesis might be induced by dysregulation of corpus cavernosum smooth muscle cells. UC-MSCs are multipotent cells that attract considerable interest due to immunoregulatory properties and might be a potential strategy to regulate and recover the functional cells and tissues, including tissue improvement in DMED.

Objective: This study aims to determine the efficacy of UC-MSCs in improving the erectile function of DMED rats through analyzing the expression of TGF-β, α-SMA, and collagen.

Methods: Total number of 30 male Sprague-Dawley rats (6 to 8 weeks old) were randomly divided into four groups (negative control group, positive control group, T1 group, and T2 group). After 16 h fast, 24 rats were randomly selected and intraperitoneally injected with streptozotocin to induce DM. At 8 weeks after STZ injection, rats with DMED were identified by unresponsive erectile stimulation within 30 min. PC group received 500 μL; T1 rats treated with 500 μL PBS containing 1x106 UC-MSCs; T2 rats treated with 500 μL PBS containing 3x106 UC-MSCs. After MSCs treatment, the rats were sacrificed and the corpus cavernosum tissues were prepared for histological observations.

Results: This study resulted in the administration of UC-MSCs could downregulate the expression of TGF-β, α-SMA, and collagen leading to the improvement of DMED.

Conclusion: UC-MSCs improve the expression of TGF-β, α-SMA, and collagen on erectile dysfunction in streptozotocin-induced diabetic rats.

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脐带来源的间充质干细胞改善糖尿病大鼠勃起功能障碍的TGF-β、α-SMA和胶原。

背景：勃起功能障碍（ED）是男性糖尿病（DM）常见的合并症之一，其发病机制可能是由海绵体平滑肌细胞失调引起的。UC MSCs是一种多能细胞，由于其免疫调节特性而备受关注，可能是一种潜在的调节和恢复功能细胞和组织的策略，包括DMED中的组织改善。目的：本研究旨在通过分析TGF-β、α-SMA、，和胶原蛋白。方法：将30只雄性Sprague-Dawley大鼠（6-8周龄）随机分为四组（阴性对照组、阳性对照组、T1组和T2组）。禁食16h后，随机选择24只大鼠，腹膜内注射链脲佐菌素诱导DM。在STZ注射后8周，通过在30min内无反应的勃起刺激鉴定DMED大鼠。PC组接受500μL；用含有1x106 UC MSCs的500μL PBS处理的T1大鼠；用含有3x106个UC MSCs的500μL PBS处理T2大鼠。MSCs治疗后，处死大鼠，制备海绵体组织进行组织学观察。结果：UC MSCs可下调TGF-β、α-SMA和胶原的表达，从而改善糖尿病大鼠的勃起功能障碍。

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