Corn peptides attenuate non-alcoholic fatty liver disease via PINK1/Parkin-mediated mitochondrial autophagy.

IF 3.5 4区 医学 Q2 FOOD SCIENCE & TECHNOLOGY Food & Nutrition Research Pub Date : 2023-09-29 eCollection Date: 2023-01-01 DOI:10.29219/fnr.v67.9547
Zhicui Yao, Xiaoling Li, Wentao Wang, Peng Ren, Shiming Song, Haiyue Wang, Ying Xie, Xingbo Li, Zengning Li
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引用次数: 1

Abstract

Background: Corn peptides, a novel food prepared from corn gluten meal (CGM) by enzymatic hydrolysis or microbial fermentation, have attracted considerable interest owing to their various bioactive properties. However, the underlying mechanism of corn peptides attenuate non-alcoholic fatty liver disease (NAFLD) remains unclear.

Objective: This study aimed to investigate the effect of corn peptides in NAFLD and to decipher the underlying mechanisms.

Design: NAFLD was induced by a high-fat diet (HFD) for 10 weeks. Corn peptides were administered during the period. Human hepatocellular carcinomas (HepG2) cells induced by free fatty acids were used for this mechanism study.

Results: Corn peptides alleviated HFD-induced histopathological changes, disorders of lipid metabolism, and mitochondrial damage. Moreover, corn peptides blocked mitophagy suppression by HFD based on the increased LC3, ATG7 expressions, as well as decreased P62 levels. Corn peptides increased the expression of proteins involved in fatty acid β-oxidation, such as PPARα and PGC-1α. Corn peptides also improved the Ser/Thr kinase PINK1 (PINK1) and the E3 ubiquitin ligase Parkin (Parkin) translocation to mitochondria, which is confirmed by immunofluorescence. Furthermore, stable knockdown of PINK1 by PINK1 SiRNA in HepG2 inhibited PINK1-Parkin-associated mitophagy and resulted in lipid accumulation.

Conclusion: Corn peptides improved cell injury and ameliorated mitochondrial dysfunction and lipid accumulation via PINK1/Parkin-mediated autophagy in NAFLD. Thus, corn peptides could be a promising nutritional molecule with natural functions for preventing NAFLD.

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玉米肽通过PINK1/Parkin介导的线粒体自噬减轻非酒精性脂肪肝。
背景:玉米肽是以玉米蛋白粉为原料,经酶解或微生物发酵制备的一种新型食品,由于其具有多种生物活性,引起了人们的极大兴趣。然而,玉米肽减轻非酒精性脂肪肝(NAFLD)的潜在机制尚不清楚。目的:本研究旨在探讨玉米肽对NAFLD的影响,并揭示其潜在机制。设计:通过高脂肪饮食(HFD)诱导NAFLD达10周。在此期间施用玉米肽。利用游离脂肪酸诱导的人肝癌细胞(HepG2)进行了该机制的研究。结果:玉米肽减轻了HFD引起的组织病理学变化、脂质代谢紊乱和线粒体损伤。此外,玉米肽基于LC3、ATG7表达的增加以及P62水平的降低阻断了HFD对线粒体自噬的抑制。玉米肽增加了参与脂肪酸β-氧化的蛋白质的表达,如PPARα和PGC-1α。玉米肽还改善了Ser/Thr激酶PINK1(PINK1)和E3泛素连接酶Parkin(Parkin)向线粒体的易位,这一点已通过免疫荧光得到证实。此外,在HepG2中,PINK1-SiRNA稳定敲低PINK1抑制了PINK1-Parkin相关的线粒体自噬,并导致脂质积聚。结论:玉米肽通过PINK1/Parkin介导的NAFLD自噬改善细胞损伤,改善线粒体功能障碍和脂质积累。因此,玉米肽可能是一种具有天然预防NAFLD功能的有前景的营养分子。
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来源期刊
Food & Nutrition Research
Food & Nutrition Research FOOD SCIENCE & TECHNOLOGY-NUTRITION & DIETETICS
CiteScore
5.20
自引率
9.10%
发文量
47
审稿时长
14 weeks
期刊介绍: Food & Nutrition Research is a peer-reviewed journal that presents the latest scientific research in various fields focusing on human nutrition. The journal publishes both quantitative and qualitative research papers. Through an Open Access publishing model, Food & Nutrition Research opens an important forum for researchers from academic and private arenas to exchange the latest results from research on human nutrition in a broad sense, both original papers and reviews, including: * Associations and effects of foods and nutrients on health * Dietary patterns and health * Molecular nutrition * Health claims on foods * Nutrition and cognitive functions * Nutritional effects of food composition and processing * Nutrition in developing countries * Animal and in vitro models with clear relevance for human nutrition * Nutrition and the Environment * Food and Nutrition Education * Nutrition and Economics Research papers on food chemistry (focus on chemical composition and analysis of foods) are generally not considered eligible, unless the results have a clear impact on human nutrition. The journal focuses on the different aspects of nutrition for people involved in nutrition research such as Dentists, Dieticians, Medical doctors, Nutritionists, Teachers, Journalists and Manufacturers in the food and pharmaceutical industries.
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