The effect of hypoxia on Hydrogen Sulfide concentration of brain tissue in AD transgenic mice and its mechanism.

Neuro endocrinology letters Pub Date : 2023-07-05

Xiumei Cheng, Meixia Ding, Churong Wei, Feng Xiao, Jiawan Liao, Mengjun Li, Zhiyong Xiao, Xiaoai Liu, Yougen Luo

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Abstract

Objective: Research has shown that hydrogen sulfide (H2S) plays a protective role in many diseases of the nervous system. The aim of this study is to investigate the effect of hypoxia on endogenous H2S concentration in the cerebral cortex of Alzheimer's disease (AD) transgenic mice and its mechanism.

Methods: AD transgenic mice were raised in closed boxes and pure nitrogen was introduced to reduce the oxygen concentration to 8%-10%, establishing an animal model of hypoxia. Oxygen partial pressure was measured with an oxygen meter. The expression of cystathionine-β-synthase (CBS) in cerebral cortex tissue was determined by Western blot, and H2S concentration was measured by a modified methylene blue method.

Results: (1) Hypoxia down-regulated CBS expression in cerebral cortex tissue of AD transgenic mice (p < 0.05). (2) The concentration of H2S in the cerebral cortex tissue of the hypoxic transgenic group was significantly lower than that of the Control group (p < 0.01). (3) Overexpression of CBS reversed the hypoxia-induced decrease of H2S concentration in the cerebral cortex tissue of AD transgenic mice (p < 0.01).

Conclusions: Hypoxia decreased the concentration of endogenous H2S in the cerebral cortex tissue of AD transgenic mice by down-regulating the expression of CBS.

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缺氧对AD转基因小鼠脑组织硫化氢浓度的影响及其机制。

目的：研究表明，硫化氢（H2S）对多种神经系统疾病具有保护作用。本研究旨在探讨缺氧对阿尔茨海默病（AD）转基因小鼠大脑皮层内源性H2S浓度的影响及其机制。方法：将AD转基因小鼠关箱饲养，引入纯氮使氧浓度降至8%-10%，建立缺氧动物模型。用氧气计测量氧气分压。Western印迹法检测大脑皮层组织中胱硫醚-β-合酶（CBS）的表达，改良亚甲蓝法测定H2S浓度。结果：（1）缺氧可下调AD转基因小鼠大脑皮层组织CBS的表达（p<0.05）；（2）缺氧转基因组大脑皮层组织中H2S浓度明显低于对照组（p<0.01）结论：缺氧通过下调CBS的表达，降低了AD转基因小鼠大脑皮层组织中内源性H2S的浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Neuro endocrinology letters

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