Biclonal Low-Grade B Cell Lymphoma as Detected by Multiparametric Flow Cytometry in Blood and Marrow.

IF 0.7 4区医学 Q4 HEMATOLOGY Indian Journal of Hematology and Blood Transfusion Pub Date : 2023-10-01 Epub Date: 2023-01-02 DOI:10.1007/s12288-022-01625-y

Thulasi Raman Ramalingam, Srikanth Muralikrishnan, Jose Easow, Lakshman Vaidhyanathan

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Abstract

Biclonal B cell lymphomas (BCL) of two different low-grade components are rare. Multiparametric flow cytometry (MFC) is an integral tool in lymphoma diagnosis and very efficient in picking up BCL cases, especially when the associated second component is small. We studied the incidence of BCL in the routine blood and marrow samples sent for immunophenotyping by MFC. A total of 376 cases were analyzed retrospectively. We studied their clinical, immunophenotypic, molecular, and cytogenetic findings with literature review. We found five cases of BCL with two different clonal low-grade B cell components at an incidence of 1.3%. All cases were males with a median age of 68 years. The chronic lymphocytic leukemia (CLL) clone was present in all 5 cases. The other associated component in cases 1 to 5 were lymphoplasmacytic lymphoma, follicular lymphoma, splenic marginal zone lymphoma, hairy cell leukemia, and monoclonal B cell population of non-CLL type respectively. Diagnosing BCL may be challenging and may also go undiagnosed when the second component is petite and overshadowed by the more significant component. It may be critical if the small, unnoticed lymphoma component is of a more aggressive type.

Supplementary information: The online version contains supplementary material available at 10.1007/s12288-022-01625-y.

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血液和骨髓多参数流式细胞术检测双克隆低级别B细胞淋巴瘤。

两种不同级别成分的双克隆B细胞淋巴瘤（BCL）是罕见的。多参数流式细胞术（MFC）是淋巴瘤诊断中不可或缺的工具，在发现BCL病例方面非常有效，尤其是当相关的第二成分很小时。我们研究了通过MFC进行免疫表型分析的常规血液和骨髓样本中BCL的发生率。对376例病例进行回顾性分析。我们通过文献综述研究了他们的临床、免疫表型、分子和细胞遗传学发现。我们发现5例BCL具有两种不同的克隆性低级别B细胞成分，发病率为1.3%。所有病例均为男性，中位年龄为68岁。慢性淋巴细胞白血病（CLL）克隆存在于所有5例病例中。病例1-5中的其他相关成分分别是淋巴浆细胞淋巴瘤、滤泡性淋巴瘤、脾边缘区淋巴瘤、毛细胞白血病和非CLL型单克隆B细胞群。诊断BCL可能很有挑战性，当第二个成分较小并被更重要的成分掩盖时，也可能无法诊断。如果小的、未被注意到的淋巴瘤成分是一种更具侵袭性的类型，这可能是至关重要的。补充信息：在线版本包含补充材料，请访问10.1007/s12288-022-01625-y。

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来源期刊

Indian Journal of Hematology and Blood Transfusion HEMATOLOGY-

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Indian Journal of Hematology and Blood Transfusion is a medium for propagating and exchanging ideas within the medical community. It publishes peer-reviewed articles on a variety of aspects of clinical hematology, laboratory hematology and hemato-oncology. The journal exists to encourage scientific investigation in the study of blood in health and in disease; to promote and foster the exchange and diffusion of knowledge relating to blood and blood-forming tissues; and to provide a forum for discussion of hematological subjects on a national scale. The Journal is the official publication of The Indian Society of Hematology & Blood Transfusion.