The combination of natural compounds Crila and epigallocatechin gallate showed enhanced antiproliferative effects on human uterine fibroid cells compared with single treatments

F&S science Pub Date : 2023-11-01 DOI:10.1016/j.xfss.2023.09.004

Tao Bai Ph.D. , Mohamed Ali Ph.D. , Bernard Somers B.S. , Qiwei Yang Ph.D. , Sue McKinney J.D. , Ayman Al-Hendy M.D., Ph.D.

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Abstract

Objective

To investigate the combined effects of Crila and green tea extract, epigallocatechin gallate (EGCG), compared with single treatments, on human uterine fibroid cells.

Design

Human uterine leiomyoma (HuLM) cells were treated with different concentrations of Crila, alone or in combination with EGCG, and several experiments were employed.

Setting

A laboratory study.

Patientss

N/A.

Interventions

Crila, EGCG.

Main Outcome Measures

Cell proliferation assay, drug synergy using combination index, protein and gene expression analysis of proliferation marker proliferating cell nuclear antigen, and apoptosis marker BAX using western blotting and quantitative polymerase chain reaction, respectively.

Results

Results showed that tested Crila concentrations, when combined with 25 and 50 μM EGCG, exerted synergistic growth inhibitory effects on HuLM viability. This inhibitory effect on HuLM cell viability was because of decreased cell proliferation, as shown by a decrease in the proliferation marker proliferating cell nuclear antigen at messenger RNA and protein levels, rather than inducing apoptosis.

Conclusion

Our study concludes that the utility of natural compounds may provide a safe and cost-effective alternative to currently used short-term hormonal therapies against uterine fibroids.

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与单一治疗相比，天然化合物Crila和表没食子儿茶素没食子酸盐（EGCG）的组合对人类子宫肌瘤细胞的抗增殖作用增强。

目的：研究红樱桃和绿茶提取物表没食子儿茶素没食子酸酯（EGCG）对人子宫肌瘤细胞的联合作用，并与单次治疗进行比较。设计：用不同浓度的Crila单独或与EGCG联合处理人子宫平滑肌瘤（HuLM）细胞，并进行了几个实验。设置：实验室研究患者（S）：不适用。干预措施：Crila，EGCG主要观察指标（S），细胞增殖测定，使用组合指数的药物协同作用，分别使用蛋白质印迹和qPCR对增殖标志物PCNA和凋亡标志物BAX的蛋白质和基因表达进行分析。结果：结果表明，当与25和50μM EGCG联合使用时，测试的Crila浓度对HuLM的活力具有协同生长抑制作用。这种对HuLM细胞活力的抑制作用是由于细胞增殖减少，如增殖标记物PCNA在mRNA和蛋白质水平上的减少所示，而不是诱导细胞凋亡。结论：我们的研究得出结论，天然化合物的效用可能为目前使用的针对不明飞行物的短期激素疗法提供一种安全且具有成本效益的替代方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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