The effect of sepsis and reactive oxygen species on skeletal muscle interstitial oxygen pressure during contractions

IF 1.9 4区医学 Q3 HEMATOLOGY Microcirculation Pub Date : 2023-10-06 DOI:10.1111/micc.12833

Naoki Hitosugi, Kazuki Hotta, Yoshikazu Taketa, Ren Takamizawa, Yutaka Fujii, Ryo Ikegami, Hajime Tamiya, Tatsuro Inoue, Atsuhiro Tsubaki

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Abstract

Objective

This study aims to examine the effect of sepsis on the dynamics of skeletal muscle partial oxygen pressure during muscle contractions as well as the effect of reactive oxygen species (ROS) scavenger (ascorbic acid, Asc).

Methods

Twenty-seven male Sprague–Dawley rats (2–3 months old) were randomly assigned to three groups; sham, cecal ligation and puncture (CLP), or CLP plus ascorbic acid treatment group (CLP + Asc). Electrical stimuli-induced muscle contractions and partial oxygen pressure measurements were performed at 3 h after CLP. The interstitial oxygen pressure (PO₂is) in the spinotrapezius muscle was measured by the phosphorescence quenching method.

Results

The PO₂is at rest was not different between the three groups. The PO₂is decreased from rest to contraction in all groups. Compared to the sham, the time to decrease PO₂is was significantly faster in CLP but not in CLP + Asc (p < .05). Compared to the sham, the PO₂is during muscle contractions was significantly lower in both CLP and CLP + Asc (p < .05, respectively).

Conclusions

Our results suggest that CLP-induced sepsis accelerated the decay of PO₂is at the onset of muscle contractions and maintained a low level of PO₂is during muscle contractions.

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败血症和活性氧对收缩过程中骨骼肌间质氧压的影响。

目的：本研究旨在探讨败血症对肌肉收缩过程中骨骼肌氧分压动力学的影响以及活性氧清除剂（抗坏血酸，Asc）的影响。方法：27只雄性Sprague-Dawley大鼠（2-3只）月龄）随机分为三组；假手术、盲肠结扎穿刺（CLP）或CLP加抗坏血酸治疗组（CLP + Asc）。电刺激引起的肌肉收缩和氧分压测量在3 CLP后h。用磷光猝灭法测定了脊髓斜方肌间质氧压。结果：三组患者PO2静息状态差异无统计学意义。在所有组中，PO2从静止到收缩都有所下降。与假手术相比，CLP中降低PO2 is的时间明显更快，但CLP中没有 + Asc（p 在CLP和CLP的肌肉收缩过程中，2 is显著降低 + Asc（p 结论：我们的研究结果表明，CLP诱导的败血症在肌肉收缩开始时加速了PO2-is的衰变，并在肌肉收缩期间保持了低水平的PO2-is。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.