Hussam A. Al Saoud, Michał Szumski, Myroslav Sprynskyy, Szymon Bocian, Bogusław Buszewski
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Abstract
The aim of this work was to use the biosilica obtained from diatoms (microalgae) cultivated under laboratory conditions as a new stationary phase to fill the chromatographic column and test it in high-performance liquid chromatography. Biosilica is an inorganic polymer consisting of orthosilicate units formed by organisms such as diatoms or siliceous sponges. The results showed that the prepared columns were characterized by relatively high efficiency, comparable to a commercially available material HALO HILIC of dp = 2.7 μm. The retention of polar compounds under a high acetonitrile content was worse than that on HALO HILIC, but it was proportionally lower when the surface area of both materials was compared. As model test solutes, nucleosides and nucleobases have undergone testing. They were examined separately for retention, and attempts to separate test mixtures were successful.
期刊介绍：
Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.
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