Pea aphid odorant-binding protein ApisOBP6 discriminates between aphid sex pheromone components, aphid alarm pheromone and a host plant volatile

IF 3.2 2区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Biochemistry and Molecular Biology Pub Date : 2023-11-01 DOI:10.1016/j.ibmb.2023.104026
Cassie Sims , Michael A. Birkett , Neil J. Oldham , Robert A. Stockman , David M. Withall
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Abstract

Olfactory perception of pheromones in insects involves odorant-binding proteins (OBPs), relatively small proteins (ca.110-240 amino acid residues) that can bind reversibly to behaviourally active olfactory ligands. In this study, we investigated the binding in silico and in vitro of the aphid sex pheromone components (1R,4aS,7S,7aR)-nepetalactol and (4aS,7S,7aR)-nepetalactone and the aphid alarm pheromone (E)-β-farnesene by OBPs from the pea aphid, Acyrthosiphon pisum. Screening of protein models of ApisOBPs1-11 with the aphid sex pheromone components suggested that ApisOPB6 was a candidate. Fluorescence assays using ApisOBP6 suggested that ApisOBP6 was able to bind both sex pheromone components and discriminate from the aphid alarm pheromone and the generic plant compound (R/S)-linalool. Saturation transfer difference NMR experiments with ApisOBP6 yielded results consistent to those from the fluorescence experiments, with a clear interaction between ApisOBP6 and (4aS,7S,7aR)-nepetalactone. These results describe a novel interaction and potential function for ApisOBP6, point to pre-receptor odorant discrimination by OBPs, and provide a platform for investigating the function of other aphid olfactory proteins involved in aphid chemical ecology.

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豌豆蚜气味结合蛋白OBP6可区分蚜虫性信息素组分、蚜虫报警信息素和寄主植物挥发物。
昆虫对信息素的嗅觉感知涉及气味结合蛋白(OBPs),这是一种相对较小的蛋白质(约110至240个氨基酸残基),可以与行为活性嗅觉配体可逆结合。在本研究中,我们在计算机和体外研究了豌豆蚜性信息素成分(1R,4aS,7S,7aR)-nepetalactol和(4aS,6S,7aR)-nepeetalone与蚜虫警报信息素(E)-β-法尼烯的结合。用蚜虫性信息素组分筛选ApisOBPs1-11的蛋白质模型表明ApisOBB6是一个候选者。使用ApisOBP6的荧光分析表明,ApisOBC6能够结合性信息素组分,并与蚜虫报警信息素和普通植物化合物芳樟醇区分开来。ApisOBP6的饱和转移差(STD)-NMR实验产生的结果与荧光实验的结果一致,ApisOBC6和(4aS,7S,7aR)-尼泊尔内酯之间存在明显的相互作用。这些结果描述了ApisOBP6的一种新的相互作用和潜在功能,指出了OBPs对受体前气味物质的识别,并为研究其他参与蚜虫化学生态学的嗅觉蛋白的功能提供了平台。
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来源期刊
CiteScore
7.40
自引率
5.30%
发文量
105
审稿时长
40 days
期刊介绍: This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.
期刊最新文献
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