Molecular Identification of Aedes, Armigeres, and Culex Mosquitoes (Diptera: Culicidae) Using Mitochondrial Cytochrome Oxidase Subunit I Genes in Myanmar

IF 1.2 3区农林科学 Q4 PARASITOLOGY Acta Parasitologica Pub Date : 2023-10-13 DOI:10.1007/s11686-023-00721-x

Si Thu Aung, Saw Bawm, Hla Myet Chel, May June Thu, Soe Soe Wai, Yuki Eshita, Ryo Nakao, Ken Katakura, Lat Lat Htun

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Abstract

Purpose

Mosquitoes are important vectors that carry disease-causing agents that can affect humans and animals. DNA barcoding is a complementary identification which can be used to validate morphological characterization of mosquito species. The objectives of this study were to identify the mitochondrial sequence of the COI gene and to construct a molecular phylogeny based on the genetic divergence of the mosquito species studied.

Methods

In this study, DNA extraction and the amplification of the mitochondrial cytochrome oxidase subunit I genes (COI) were performed on pooled mosquito samples collected in Nay Pyi Taw area, Myanmar.

Results

Fragments of the COI gene showed 99–100% identity with sequences of Aedes aegypti, Armigeres subalbatus, Culex pipiens complex, and Cx. quinquefasciatus, respectively, deposited in GenBank. This study categorized two haplotypes from each Ar. subalbatus and Cx. pipiens complex COI gene sequence, as well as three haplotypes from Cx. quinquefasciatus COI gene sequences. The highest haplotype diversity and nucleotide diversity were observed in the Ar. subalbatus population (Hd = 1.0000; π = 0.0033), followed by the Cx. pipiens complex and Cx. quinquefasciatus populations.

Conclusion

This study provides useful information on the molecular identification and genetic diversity of mosquito vectors with medical and veterinary significance, which may assist in the improvement of mosquito control programs.

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利用缅甸线粒体细胞色素氧化酶亚基I基因对伊蚊、军蚊和库蚊的分子鉴定（直翅目：库蚊科）。

目的：蚊子是携带致病因子的重要媒介，可影响人类和动物。DNA条形码是一种互补的鉴定方法，可用于验证蚊子物种的形态特征。本研究的目的是鉴定COI基因的线粒体序列，并根据所研究蚊子物种的遗传差异构建分子系统发育。方法：在本研究中，对缅甸内比都地区采集的蚊子样本进行了线粒体细胞色素氧化酶亚基I基因（COI）的DNA提取和扩增。结果：COI基因片段与GenBank中埃及伊蚊（Aedes aegypti。本研究分类了来自每个Ar.subalbatus和Cx.pipiens复合物COI基因序列的两个单倍型，以及来自Cx.致倦库蚊COI基因顺序的三个单倍型。在Ar.subalbatus群体中观察到最高的单倍型多样性和核苷酸多样性（Hd = 1.0000；π = 0.0033），其次是Cx.pipiens复合体和Cx.致倦库蚊种群。结论：本研究为蚊虫媒介的分子鉴定和遗传多样性提供了有用的信息，具有医学和兽医意义，有助于改进蚊虫控制方案。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.