Simple, low-cost, and well-performing method, the outgrowth technique, for the isolation of cells from nasal polyps.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-10-10 DOI:10.1186/s12860-023-00493-2

Jonghui Kim, Karla Hegener, Claudia Hagedorn, Daniel Weidinger, Kashin Jamal Jameel, Inga Marte Charlott Seuthe, Sabine Eichhorn, Florian Kreppel, Jonas Jae-Hyun Park, Jürgen Knobloch

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Abstract

Background: Epithelial cells are an important part of the pathomechanism in chronic rhinosinusitis with nasal polyps. It is therefore essential to establish a robust method for the isolation and culture of epithelial cells from nasal polyps to enable further research. In this study, the feasibility of the outgrowth technique for the isolation of the epithelial cells from the nasal polyps was evaluated.

Results: Using the outgrowth technique, epithelial cells could be isolated from all tissue samples. Isolated epithelial cells showed a proliferation rate of approximately 7- to 23-fold every 6 days up to the 3rd passage. Over 97% of isolated cells were shown to be cytokeratin- and p63-positive, and over 86% of them were Ki-67-positive in flow cytometry. Interleukin-33 and periostin were detectable in the supernatant.

Conclusions: We introduce a simple, low-cost, and well-performing method for isolating epithelial cells from nasal polyps with the outgrowth technique.

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从鼻息肉中分离细胞的简单、低成本、性能良好的方法，即生长技术。

背景：上皮细胞是慢性鼻窦炎合并鼻息肉发病机制的重要组成部分。因此，有必要建立一种强有力的方法来分离和培养鼻息肉上皮细胞，以便进行进一步的研究。在本研究中，评估了从鼻息肉中分离上皮细胞的生长技术的可行性。结果：利用生长技术可以从所有组织样本中分离出上皮细胞。直到第3代，分离的上皮细胞显示出大约每6天7至23倍的增殖率。在流式细胞术中，超过97%的分离细胞显示为细胞角蛋白和p63阳性，其中超过86%的细胞为Ki-67阳性。上清液中可检测到白细胞介素-33和紫苏素。结论：我们介绍了一种简单、低成本、高效的鼻息肉生长技术分离鼻息肉上皮细胞的方法。

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