Anticancer effects of gossypetin from Hibiscus sabdariffa in oral squamous cell carcinoma.

IF 2.2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Applied Oral Science Pub Date : 2023-10-09 eCollection Date: 2023-01-01 DOI:10.1590/1678-7757-2023-0243

Ke Huang, Zhibin Liu, Myoung-Ok Kim, Ki-Rim Kim

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Abstract

Objective: Gossypetin, isolated from Hibiscus sabdariffa L, has been shown to have various pharmacological effects including anti-inflammatory and antibacterial activity against various diseases. However, since the effect of gossypetin in oral cancer remains to be reported, we aimed to investigate the anticancer activity and mechanisms of gossypetin in oral squamous cell carcinoma (OSCC).

Methodology: The proliferation of OSCC cells was evaluated by cell viability and soft agar colony assays. The effects of gossypetin on the migration and invasion of OSCC cells was investigated by wound healing and transwell invasion assays, respectively. Apoptosis and cell cycle arrest were measured by flow cytometry. Moreover, the anticancer mechanism of gossypetin in OSCC cells was analyzed by western blotting.

Results: Gossypetin inhibited the proliferation, migration, and invasion of OSCC cells and induced apoptosis by upregulating the Bax/Bcl-2 ratio and cell cycle arrest at the G2/M phase. Furthermore, gossypetin regulated the activation of extracellular signal-regulated kinase and nuclear factor-kappa B.

Conclusion: Results showed that gossypetin inhibits the proliferation, migration, and invasion of OSCC cells and triggers apoptosis and cell cycle arrest in OSCC. Therefore, gossypetin has the potential for use as a chemopreventive agent in oral cancer.

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木槿棉子素对口腔鳞状细胞癌的抗癌作用。

目的：从木槿中分离得到的棉素具有多种药理作用，包括对各种疾病的抗炎和抗菌活性。然而，由于棉子素在口腔癌症中的作用尚待报道，我们旨在研究棉子素对口腔鳞状细胞癌（OSCC）的抗癌活性和机制。方法：通过细胞活力和软琼脂集落测定来评估OSCC细胞的增殖。分别通过伤口愈合和transwell侵袭试验研究了棉子素对OSCC细胞迁移和侵袭的影响。流式细胞仪检测细胞凋亡和细胞周期阻滞。此外，通过蛋白质印迹分析了棉子素在OSCC细胞中的抗癌机制。结果：Gossypetin通过上调Bax/Bcl-2比值和细胞周期停滞于G2/M期，抑制OSCC细胞的增殖、迁移和侵袭，并诱导细胞凋亡。此外，棉子素还调节细胞外信号调节激酶和核因子κB的激活。结论：棉子素抑制OSCC细胞的增殖、迁移和侵袭，并触发OSCC细胞凋亡和细胞周期停滞。因此，棉子素具有作为口腔癌症化学预防剂的潜力。

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来源期刊

Journal of Applied Oral Science 医学-牙科与口腔外科

CiteScore

4.80

自引率

3.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.