Draft genome sequences for three unisolated Alnus-infective Frankia Sp+ strains, AgTrS, AiOr and AvVan, the first sequenced Frankia strains able to sporulate in-planta.

Journal of Genomics Pub Date : 2019-09-17 eCollection Date: 2019-01-01 DOI:10.7150/jgen.35875
Lorine Bethencourt, Florian Vautrin, Najwa Taib, Audrey Dubost, Lucia Castro-Garcia, Olivier Imbaud, Danis Abrouk, Pascale Fournier, Jérôme Briolay, Agnès Nguyen, Philippe Normand, Maria P Fernandez, Céline Brochier-Armanet, Aude Herrera-Belaroussi
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引用次数: 7

Abstract

Actinobacteria from genus Frankia are able to form symbiotic associations with actinorhizal plants including alders. Among them, Sp+ strains are characterized by their ability to differentiate numerous sporangia inside host plant cells (unlike "Sp-" strains unable of in-planta sporulation). Here, we report the first genome sequences of three unisolated Sp+ strains: AgTrS, AiOr and AvVan obtained from Alnus glutinosa, A. incana and A. alnobetula (previously known as viridis), respectively (with genome completeness estimated at more than 98%). They represent new Frankia species based on Average Nucleotide Identity (ANI) calculations, and the smallest Alnus-infective Frankia genomes so far sequenced (~5 Mbp), with 5,178, 6,192 and 5,751 candidate protein-encoding genes for AgTrS, AiOr and AvVan, respectively.

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三株未分离的Alnus感染性Frankia Sp+菌株AgTrS、AiOr和AvVan的基因组序列草案,这是第一株能够在植物中产孢的已测序Frankia菌株。
Frankia属的放线菌能够与包括赤杨在内的放线植物形成共生关系。其中,Sp+菌株的特征是它们能够分化宿主植物细胞内的大量孢子囊(与不能在植物内形成孢子的“Sp-”菌株不同)。在这里,我们报道了三个未分离的Sp+菌株的第一个基因组序列:分别从Alnus glutinosa、A.incana和A.alnobetula(以前称为viridis)获得的AgTrS、AiOr和AvVan(基因组完整性估计超过98%)。它们代表了基于平均核苷酸同一性(ANI)计算的新Frankia物种,以及迄今为止测序的最小的Alnus感染Frankia基因组(~5Mbp),分别有5178、6192和5751个AgTrS、AiOr和AvVan的候选蛋白编码基因。
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来源期刊
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0.00%
发文量
11
审稿时长
12 weeks
期刊介绍: Journal of Genomics publishes papers of high quality in all areas of gene, genetics, genomics, proteomics, metabolomics, DNA/RNA, computational biology, bioinformatics, and other relevant areas of research and application. Articles published by the journal are rigorously peer-reviewed. Types of articles include: Research paper, Short research communication, Review or mini-reviews, Commentary, Database, Software.
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