Phylogenetic analysis of prospective M. bovis antigens with the aim of developing candidate vaccines for bovine tuberculosis.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-10-12 DOI:10.1186/s43141-023-00552-3
Zhandos Abay, Sandugash Sadikalieva, Kamshat Shorayeva, Bolat Yespembetov, Makpal Sarmykova, Kuanish Jekebekov, Gaukhar Tokkarina, Zharkinay Absatova, Elina Kalimolda, Yeraly Shayakhmetov, Sabina Moldagulova, Aisha Issabek, Syrym Kopeyev, Alisher Omurtay, Kainar Barakbayev, Markhabat Kassenov, Nazym Syrym, Yergali Abduraimov, Kunsulu Zakarya, Ainur Nurpeisova
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Abstract

Background: Bovine Tuberculosis is a respiratory disease caused by the pathogen Mycobacterium bovis (M. bovis) that infects cattle. Though rare, this disease can also affect humans, as well as domestic and wild animals, making it a serious concern. Therefore, searching for alternative and new vaccines with high efficiency and safety is the main goal in bovine tuberculosis prophylaxis. New vaccines, known as vector vaccines, have the potential to become safe and effective alternatives to the traditional BCG vaccine. In this study, two major immunodominant proteins of M. bovis Esat-6 and TB10.4 were utilized to create a vector vaccine for bovine tuberculosis.

Methods: The Esat-6 and TB10.4 genes were amplified by PCR. The amplified and purified PCR products were sequenced by the Sanger method. Assembly and multiple alignments of amplicon nucleotides were carried out in the MEGA 11 software.

Result: Two genes of the local strain 0078-M. bovis-8/RIBSP were sequenced. The nucleotide sequences were deposited in the GenBank database. Comparative analysis of the nucleotide sequences of the ESAT-6 and TB10.4 genes established 100% identity of the compared strains of Mycobacterium.

Conclusion: Through the use of phylogenetic analysis, it has been confirmed that the amplified genes are related to the mycobacteria genus. This discovery allows the development of a vector vaccine against bovine tuberculosis utilising these genes.

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牛分枝杆菌潜在抗原的系统发育分析,目的是开发牛结核病的候选疫苗。
背景:牛结核病是一种由牛分枝杆菌引起的呼吸道疾病。这种疾病虽然罕见,但也会影响人类、家畜和野生动物,使其成为一个严重的问题。因此,寻找高效、安全的替代新疫苗是预防牛结核病的主要目标。被称为载体疫苗的新疫苗有可能成为传统BCG疫苗的安全有效的替代品。在本研究中,利用牛分枝杆菌Esat-6和TB10.4的两种主要免疫显性蛋白来制备牛结核病的载体疫苗。方法:采用聚合酶链式反应扩增Esat-6和TB10.4基因。用Sanger法对扩增和纯化的PCR产物进行测序。扩增子核苷酸的组装和多重比对在MEGA 11软件中进行。结果:获得本地菌株0078-M的两个基因。对bovis-8/RIBSP进行测序。核苷酸序列存储在GenBank数据库中。ESAT-6和TB10.4基因的核苷酸序列的比较分析确定了分枝杆菌的比较菌株的100%同一性。结论:通过系统发育分析,证实扩增的基因与分枝杆菌属有亲缘关系。这一发现允许开发利用这些基因的牛结核病载体疫苗。
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