Expression of nectin-4 in prostate cancer.

Northern clinics of Istanbul Pub Date : 2023-09-25 eCollection Date: 2023-01-01 DOI:10.14744/nci.2023.36034
Melike Ordu, Mustafa Karaaslan, Mehmet Emin Sirin, Mehmet Yilmaz
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Abstract

Objective: Nectin-4 is a transmembrane protein belonging to the nectin family of immunoglobulin-like molecules which is found in the placenta and trachea under physiological conditions and its expression has been shown in many cancer types. We aimed to investigate for the 1st time nectin-4 expression in human prostate cancer tissues.

Methods: We retrospectively analyzed the prostate pathology specimens of 82 patients who underwent initial transrectal ultrasound-guided prostate biopsy or transurethral prostate resection and were found to have atypical small acinar proliferation (ASAP) and incidentally prostate cancer. Tissue samples with prostatic cancer were used as a control for alpha-methylacyl-CoA racemase (AMACR), and benign prostatic glands in the same tissue provided the negative control. The intensity and extent of nectin-4 expression were determined microscopically using the histochemical scoring system which was defined as the product of the staining intensity (score: 0-3) and percentage of stained cells (0-100) at a given intensity.

Results: We conducted immunohistochemical analysis of nectin-4 and AMACR expression in all 82 samples. While AMACR expression was positive in prostate cancer tissues with a GS of <7 (n=24, 100%), 7 (n=18, 100%), and ≥8 (n=15, 100%), it was negative in all ASAP samples (n=25, 100%) (p<0.001). Nectin-4 expression was not detected in any of the GS <7, GS 7, or GS ≥8 samples but was found in benign prostatic gland tissues and all 25 (100%) ASAP samples (p<0.001).

Conclusion: We found that nectin-4 was not expressed in prostate cancer tissues but was expressed in ASAP-and benign prostate gland containing tissues. We believe that prospective studies with more patients and samples including radical prostatectomy materials will reveal the relationship between nectin-4 and prostate cancer more clearly.

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nectin-4在前列腺癌症中的表达。
目的:Nectin-4是一种免疫球蛋白样分子连接蛋白家族的跨膜蛋白,在生理条件下存在于胎盘和气管中,在多种癌症类型中均有表达。我们的目的是首次研究nectin-4在人前列腺癌症组织中的表达。方法:回顾性分析82例经直肠超声引导前列腺穿刺或经尿道前列腺电切术后,发现有非典型小腺泡增生(ASAP)和前列腺癌的前列腺病理标本。前列腺癌症组织样本用作α-甲基酰基-CoA外消旋酶(AMACR)的对照,同一组织中的良性前列腺提供阴性对照。使用组织化学评分系统在显微镜下测定连接蛋白-4表达的强度和程度,该评分系统被定义为在给定强度下染色强度(评分:0-3)和染色细胞百分比(0-100)的乘积。结果:我们对82例标本进行了连接蛋白-4和AMACR表达的免疫组化分析。而AMACR在前列腺癌症组织中表达阳性,GS为阳性。结论:我们发现nectin-4在前列腺癌症组织中不表达,但在ASAP和良性前列腺组织中表达。我们相信,对更多患者和样本(包括根治性前列腺切除术材料)的前瞻性研究将更清楚地揭示nectin-4与前列腺癌症之间的关系。
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