Modelling in vitro gametogenesis using induced pluripotent stem cells: a review.

IF 4 Q2 CELL & TISSUE ENGINEERING Cell Regeneration Pub Date : 2023-10-16 DOI:10.1186/s13619-023-00176-5
Maria Victoria Romualdez-Tan
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Abstract

In vitro gametogenesis (IVG) has been a topic of great interest in recent years not only because it allows for further exploration of mechanisms of germ cell development, but also because of its prospect for innovative medical applications especially for the treatment of infertility. Elucidation of the mechanisms underlying gamete development in vivo has inspired scientists to attempt to recapitulate the entire process of gametogenesis in vitro. While earlier studies have established IVG methods largely using pluripotent stem cells of embryonic origin, the scarcity of sources for these cells and the ethical issues involved in their use are serious limitations to the progress of IVG research especially in humans. However, with the emergence of induced pluripotent stem cells (iPSCs) due to the revolutionary discovery of dedifferentiation and reprogramming factors, IVG research has progressed remarkably in the last decade. This paper extensively reviews developments in IVG using iPSCs. First, the paper presents key concepts from groundwork studies on IVG including earlier researches demonstrating that IVG methods using embryonic stem cells (ESCs) also apply when using iPSCs. Techniques for the derivation of iPSCs are briefly discussed, highlighting the importance of generating transgene-free iPSCs with a high capacity for germline transmission to improve efficacy when used for IVG. The main part of the paper discusses recent advances in IVG research using iPSCs in various stages of gametogenesis. In addition, current clinical applications of IVG are presented, and potential future applications are discussed. Although IVG is still faced with many challenges in terms of technical issues, as well as efficacy and safety, novel IVG methodologies are emerging, and IVG using iPSCs may usher in the next era of reproductive medicine sooner than expected. This raises both ethical and social concerns and calls for the scientific community to cautiously develop IVG technology to ensure it is not only efficacious but also safe and adheres to social and ethical norms.

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利用诱导多能干细胞模拟体外配子发生:综述。
近年来,体外配子发生(IVG)一直是人们非常感兴趣的话题,这不仅是因为它可以进一步探索生殖细胞发育的机制,还因为它在创新医学应用中的前景,特别是在治疗不孕不育方面。体内配子发育机制的阐明激发了科学家们试图概括体外配子发生的整个过程。虽然早期的研究已经确定了IVG方法,主要使用胚胎来源的多能干细胞,但这些细胞来源的稀缺性及其使用过程中涉及的伦理问题严重限制了IVG研究的进展,尤其是在人类中。然而,由于去分化和重编程因子的革命性发现,诱导多能干细胞(iPSC)的出现,IVG研究在过去十年中取得了显著进展。本文广泛综述了使用iPSC的IVG的发展。首先,本文介绍了IVG基础研究的关键概念,包括早期的研究表明,使用胚胎干细胞的IVG方法也适用于使用iPSC。简要讨论了iPSC的衍生技术,强调了产生具有高种系传播能力的无转基因iPSC以提高IVG疗效的重要性。本文的主要部分讨论了在配子发生的各个阶段使用iPSC进行IVG研究的最新进展。此外,还介绍了IVG目前的临床应用,并对其未来的潜在应用进行了讨论。尽管IVG在技术问题以及疗效和安全性方面仍面临许多挑战,但新的IVG方法正在出现,使用iPSC的IVG可能会比预期更快地开创下一个生殖医学时代。这引发了伦理和社会关注,并呼吁科学界谨慎开发IVG技术,以确保其不仅有效而且安全,并遵守社会和伦理规范。
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来源期刊
Cell Regeneration
Cell Regeneration Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
5.80
自引率
0.00%
发文量
42
审稿时长
35 days
期刊介绍: Cell Regeneration aims to provide a worldwide platform for researches on stem cells and regenerative biology to develop basic science and to foster its clinical translation in medicine. Cell Regeneration welcomes reports on novel discoveries, theories, methods, technologies, and products in the field of stem cells and regenerative research, the journal is interested, but not limited to the following topics: ◎ Embryonic stem cells ◎ Induced pluripotent stem cells ◎ Tissue-specific stem cells ◎ Tissue or organ regeneration ◎ Methodology ◎ Biomaterials and regeneration ◎ Clinical translation or application in medicine
期刊最新文献
Application and new findings of scRNA-seq and ST-seq in prostate cancer. Beyond resorption: osteoclasts as drivers of bone formation. Subtype-specific neurons from patient iPSCs display distinct neuropathological features of Alzheimer's disease. Targeting senescent cells in aging and COVID-19: from cellular mechanisms to therapeutic opportunities. Chromatin remodeling in tissue stem cell fate determination.
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