The Efficacy of Topical Formulation Containing Ciplukan (Physalis angulata Linn.) in Modulating Interleukin-17 and Interferon Gamma Expression in Mice (Mus musculus) Psoriasis Model.

Q2 Medicine Journal of Experimental Pharmacology Pub Date : 2023-10-09 eCollection Date: 2023-01-01 DOI:10.2147/JEP.S427615

Oki Suwarsa, Hartati Purbo Dharmadji, Enny Rohmawaty, Shela Mareta, Hendra Gunawan, Reiva Farah Dwiyana, Pati Aji Achdiat, Endang Sutedja, Miranti Pangastuti

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Abstract

Background: Interleukin 17 (IL-17) and interferon gamma (IFN-γ) play a role in the pathogenesis of psoriasis vulgaris (PV). Topical corticosteroids are still utilised as first-line therapy for mild to moderate PV. However, long-term use of corticosteroid is associated with various side effects. Physalis angulata Linn. (Ciplukan) possesses anti-inflammatory properties that could serve as a potential alternative topical therapy for PV.

Objective: To assess the efficacy of topical ciplukan as an anti-inflammatory agent targeting the expression of IL-17 and IFN-γ.

Methods: Psoriasis was induced using imiquimod cream, therefore divided into five groups. Group I, the psoriasis control group, received only imiquimod cream. Groups C1 and C2 received imiquimod cream followed by a mixture of Ciplukan and vaseline in a 1:2 and 1:4 ratio, respectively. Group M, the standard therapy group, received imiquimod cream, followed by mometasone furoate cream. Lastly, group V, the vehicle group, received imiquimod cream followed by vaseline album. Expression of IL-17 and IFN-γ in mice's skin tissue was analysed using reverse transcription polymerase chain reaction (RT-PCR) after seven days of treatment.

Results: The mean expression of IL-17 in Group C1 (22.60) was significantly lower (p = 0.012) than in the psoriasis control group (23.60), and there was no significant difference (p = 0.613) in Group M (22.41). The mean expression of IFN-γ in Group C1 (26.97) and Group C2 (27.03) was also significantly lower (p = 0.026 and p = 0.026, respectively) than Group I (28.80), and there was no significant difference (p = 0.180 and p = 0.093, respectively) than Group M (26.03).

Conclusion: Expression of IL-17 and IFN-γ in the ciplukan group is lower than in the psoriasis control group, and there is no significant difference compared to the standard therapy group.

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含有Ciplukan（Physalis angulata Linn.）的局部制剂在小鼠（Mus musculus）银屑病模型中调节白细胞介素-17和干扰素γ表达的功效。

背景：白细胞介素17（IL-17）和干扰素-γ（IFN-γ）在寻常型银屑病（PV）的发病机制中发挥作用。局部皮质类固醇仍然被用作轻度至中度PV的一线治疗。然而，长期使用皮质类固醇会产生各种副作用。棱藻。（Ciplukan）具有抗炎特性，可作为PV的潜在替代局部治疗方法。目的：评估局部Ciplukan作为靶向IL-17和IFN-γ表达的抗炎剂的疗效。方法：用咪喹莫特乳膏诱导银屑病，分为五组。第一组，银屑病对照组，仅接受咪喹莫特乳膏。C1组和C2组接受咪喹莫特乳膏，然后分别以1:2和1:4的比例接受西普卢康和凡士林的混合物。M组为标准治疗组，给予咪喹莫特乳膏，随后给予糠酸莫米松乳膏。最后，第五组，即赋形剂组，接受咪喹莫特乳膏，然后接受凡士林相册。治疗7天后，使用逆转录聚合酶链式反应（RT-PCR）分析小鼠皮肤组织中IL-17和IFN-γ的表达。结果：C1组（22.60）的IL-17平均表达显著低于银屑病对照组（23.60）（p=0.012），M组（22.41）无显著差异（p=0.0613）。C1组（26.97）和C2组（27.03）的IFN-γ平均表达也显著低于I组（28.80）（分别为p=0.026和p=0.026），结论：白细胞介素17和干扰素-γ在ciplukan组的表达低于银屑病对照组，与标准治疗组相比无显著差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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