Platelet Function and Maturity and Related microRNA Expression in Whole Blood in Patients with ST-Segment Elevation Myocardial Infarction.

IF 5 2区医学 Q1 HEMATOLOGY Thrombosis and haemostasis Pub Date : 2024-03-01 Epub Date: 2023-10-16 DOI:10.1055/s-0043-1776305

Oliver Buchhave Pedersen, Anne-Mette Hvas, Leonardo Pasalic, Steen Dalby Kristensen, Erik Lerkevang Grove, Peter H Nissen

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Abstract

Background: Reduced effect of antiplatelet therapy has been reported in patients with ST-segment elevation myocardial infarction (STEMI). MicroRNAs (miRs) may influence platelet function and maturity, and subsequently the effect of antiplatelet therapy.

Objectives: We aimed to explore the association between miR expression and platelet function and maturity in patients with acute STEMI and healthy individuals.

Methods: We performed an observational study of STEMI patients admitted directly to primary percutaneous coronary intervention. Patients were treated with antiplatelet therapy according to guidelines. Within 24 hours after admission, blood samples were obtained to measure: the expression of 10 candidate miRs, platelet function markers using advanced flow cytometry, platelet aggregation, serum thromboxane B₂, and platelet maturity markers. Furthermore, blood samples from healthy individuals were obtained to determine the normal variation.

Results: In total, 61 STEMI patients and 50 healthy individuals were included. STEMI patients had higher expression of miR-21-5p, miR-26b-5p, and miR-223-3p and lower expression of miR-150-5p, miR423-5p, and miR-1180-3p than healthy individuals. In STEMI patients, the expression of miR-26b-5p showed the most consistent association with platelet function (all p-values <0.05, Spearman's rho ranging from 0.27 to 0.41), while the expression of miR-150-5p and miR-223-3p showed negative associations with platelet function. No association between miR expression and platelet maturity markers was observed.

Conclusion: In patients with STEMI, the expression of six miRs was significantly different from healthy individuals. The expression of miR-26b-5p may affect platelet function in acute STEMI patients and potentially influence the effect of antiplatelet therapy.

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ST段抬高型心肌梗死患者血小板功能、成熟度及相关microRNA在全血中的表达。

背景：据报道，抗血小板治疗对ST段抬高型心肌梗死（STEMI）患者的疗效降低。微小RNA（miRs）可能影响血小板功能和成熟度，进而影响抗血小板治疗的效果。目标：我们旨在探讨急性STEMI患者和健康人的miR表达与血小板功能和成熟度之间的关系。方法：我们对直接接受初级经皮冠状动脉介入治疗的STEMI患者进行了一项观察性研究。根据指导原则对患者进行抗血小板治疗。24小时内入院数小时后，采集血样以测量：10种候选miR的表达、使用先进流式细胞术的血小板功能标志物、血小板聚集、血清血栓素B2和血小板成熟度标志物。此外，从健康个体获得血液样本以确定正常变异。结果：总共包括61名STEMI患者和50名健康人。STEMI患者的miR-21-5p、miR-26b-5p和miR-223-3p的表达高于健康个体，miR-150-5p、iR423-5p和miR-1180-3p的表达低于健康个体。在STEMI患者中，miR-26b-5p的表达显示出与血小板功能最一致的相关性（所有p值结论：在STEMI患者中，6种miR的表达与健康个体显著不同。miR-26b-5p的表达可能影响急性STEMI患者的血小板功能，并可能影响抗血小板治疗的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Thrombosis and haemostasis 医学-外周血管病

CiteScore

11.90

自引率

9.00%

发文量

140

审稿时长

1 months

期刊介绍： Thrombosis and Haemostasis publishes reports on basic, translational and clinical research dedicated to novel results and highest quality in any area of thrombosis and haemostasis, vascular biology and medicine, inflammation and infection, platelet and leukocyte biology, from genetic, molecular & cellular studies, diagnostic, therapeutic & preventative studies to high-level translational and clinical research. The journal provides position and guideline papers, state-of-the-art papers, expert analysis and commentaries, and dedicated theme issues covering recent developments and key topics in the field.