Cell-Free PURE System: Evolution and Achievements.

Q2 Agricultural and Biological Sciences 生物设计研究(英文) Pub Date : 2022-08-30 eCollection Date: 2022-01-01 DOI:10.34133/2022/9847014
Yi Cui, Xinjie Chen, Ze Wang, Yuan Lu
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引用次数: 1

Abstract

The cell-free protein synthesis (CFPS) system, as a technical core of synthetic biology, can simulate the transcription and translation process in an in vitro open environment without a complete living cell. It has been widely used in basic and applied research fields because of its advanced engineering features in flexibility and controllability. Compared to a typical crude extract-based CFPS system, due to defined and customizable components and lacking protein-degrading enzymes, the protein synthesis using recombinant elements (PURE) system draws great attention. This review first discusses the elemental composition of the PURE system. Then, the design and preparation of functional proteins for the PURE system, especially the critical ribosome, were examined. Furthermore, we trace the evolving development of the PURE system in versatile areas, including prototyping, synthesis of unnatural proteins, peptides and complex proteins, and biosensors. Finally, as a state-of-the-art engineering strategy, this review analyzes the opportunities and challenges faced by the PURE system in future scientific research and diverse applications.

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无细胞PURE系统:进化和成就。
无细胞蛋白质合成(CFPS)系统作为合成生物学的技术核心,可以在没有完整活细胞的体外开放环境中模拟转录和翻译过程。由于其在柔性和可控性方面的先进工程特性,它在基础和应用研究领域得到了广泛的应用。与典型的基于粗提取物的CFPS系统相比,由于组分明确且可定制,并且缺乏蛋白质降解酶,使用重组元件(PURE)系统的蛋白质合成引起了极大的关注。这篇综述首先讨论了PURE系统的元素组成。然后,对PURE系统的功能蛋白,特别是关键核糖体的设计和制备进行了研究。此外,我们追踪了PURE系统在多功能领域的发展,包括原型设计、非天然蛋白质、肽和复杂蛋白质的合成以及生物传感器。最后,作为一种最先进的工程策略,本文分析了PURE系统在未来科学研究和各种应用中面临的机遇和挑战。
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CiteScore
3.90
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0.00%
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审稿时长
12 weeks
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