Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia

IF 0.3 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of the Indonesian Tropical Animal Agriculture Pub Date : 2023-02-14 DOI:10.14710/jitaa.48.1.28-36
E. T. Setiatin, D. A. Lestari, D. W. Harjanti, A. Setiaji, E. Kurnianto
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Abstract

The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
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促卵泡激素受体基因探索作为印尼当地山羊高产性状的可能标记
本研究的目的是发现和鉴定促卵泡激素受体(FSHR)基因SNP对克荷邦(KJ)和埃塔沃级(EG)母鹿多产性状的影响。从11名KJ和4名EG中采集了15份不同产次和出生类型的血样。利用聚合酶链式反应(PCR)从DNA模板中扩增出FSHR基因;然后对PCR产物进行测序以确定单核苷酸多态性(SNP)。结果表明,共鉴定出3个SNPs,这些SNPs改变了氨基酸序列,形成了6个单倍型,并根据出生类型对母鹿进行了划分。总之,本研究中鉴定的SNPs与可作为遗传标记的多产性状有关,单倍型3、4和5在KJ和EG母鹿上表现出最高的多产性。
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来源期刊
Journal of the Indonesian Tropical Animal Agriculture
Journal of the Indonesian Tropical Animal Agriculture AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
1.10
自引率
0.00%
发文量
5
审稿时长
8 weeks
期刊介绍: Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers. The journal covers all aspect relating to Animal Agriculture, including: -Animal breeding and genetics -Animal feeding and nutrition -Animal reproduction -Animal biotechnology -Animal physiology -Animal production and technology -Animal products technology -Animal management and economics -Animal products processing and animal by-products -Animal microbiology -Livestock farming systems -Other related topics in relation to animal science. The topics of research are not only on Indonesian tropical animal agriculture, but also on other tropical regions of the world.
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