Reduction in breast cancer susceptibility due to XbaI gene polymorphism of alpha estrogen receptor gene in Jordanians

IF 3.3 4区 医学 Q2 ONCOLOGY Breast Cancer : Targets and Therapy Pub Date : 2017-01-24 DOI:10.2147/BCTT.S125652
M. Atoum, Foad Alzoughool
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引用次数: 8

Abstract

Breast cancer is a global health concern among women worldwide. Estrogen receptor alpha (ERα) mediates diverse polymorphic effects in breast tissues that may relate to breast cancer susceptibility. The aim of this study was to evaluate the effect of −397 PvuII (T/C) and −351 XbaI (A/G) restriction fragment length polymorphism within intron 1 of ERα, and its effect on breast cancer susceptibility. A total of 156 women who were histopathologically diagnosed with breast cancer and 142 healthy Jordanian women were enrolled in this case–control study. Genomic DNA was extracted from whole peripheral blood, and the desired fragment was amplified using polymerase chain reaction followed by restriction digestion with PvuII and XbaI restriction enzymes. The results showed no significant association between PvuII polymorphism and breast cancer risk. However, a significant association was found between XbaI polymorphism and reduction in breast cancer risk within the “x” allele of heterozygotes (odds ratio [OR] 0.199, 95% confidence interval [CI] 0.09–0.044) and heterozygotes (OR 0.208, 95% CI 0.09–0.047). The combined analysis of PvuII and XbaI polymorphisms revealed a synergistic effect of Pp/Xx and pp/xx genotypes and a significant reduction in breast cancer risk with these genotypes. The results also showed no statistical differences among PvuII or XbaI polymorphisms based on stage, ER, progesterone receptor and expression of hormone receptor such as human epidermal growth factor receptor 2. This case–control study showed that XbaI polymorphism of alpha estrogen gene modified and reduced breast cancer susceptibility among Jordanians.
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约旦人α-雌激素受体基因XbaI基因多态性降低癌症易感性
乳腺癌是全世界妇女关注的一个全球性健康问题。雌激素受体α (ERα)在乳腺组织中介导多种多态性效应,可能与乳腺癌易感性有关。本研究旨在探讨ERα内含子1内- 397 pvii (T/C)和- 351 XbaI (A/G)限制性内切片段长度多态性对乳腺癌易感性的影响。共有156名组织病理学诊断为乳腺癌的妇女和142名健康的约旦妇女参加了这项病例对照研究。从全外周血中提取基因组DNA,用聚合酶链反应扩增所需片段,然后用PvuII和XbaI酶切酶切。结果显示PvuII多态性与乳腺癌风险之间无显著关联。然而,在杂合子的“x”等位基因中发现XbaI多态性与乳腺癌风险降低之间存在显著关联(比值比[OR] 0.199, 95%置信区间[CI] 0.09-0.044)和杂合子(OR 0.208, 95% CI 0.09-0.047)。pvii和XbaI基因多态性的联合分析显示Pp/Xx和Pp/Xx基因型具有协同效应,并且显著降低了这些基因型的乳腺癌风险。PvuII和XbaI在分期、ER、孕激素受体和激素受体(如人表皮生长因子受体2)表达方面的多态性也无统计学差异。本病例对照研究表明,雌激素基因XbaI多态性改变并降低了约旦人乳腺癌的易感性。
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来源期刊
CiteScore
4.10
自引率
0.00%
发文量
40
审稿时长
16 weeks
期刊最新文献
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