LVSEP Analysis of Cationic Analytes in Non-Aqueous Capillary Electrophoresis

Abstract

To achieve highly sensitive analyses of cationic analytes with simple experimental procedures in non-aqueous capillary electrophoresis (NACE), large-volume sample stacking with an electroosmotic flow pump (LVSEP) was performed in dynamically polymer coated capillaries. As the dynamic coating polymers, cationic polybrene (PB), and neutral polymer, (hydroxypropyl)methyl cellulose (HPMC), were employed in methanolic media to reverse and suppress the electroosmotic flow. In the analysis of cationic amines, good enrichments were attained with the sensitive enhancement factor (SEF) of 70 and 687 for benzylamine and 1-(1-naphthyl)ethylamine, respectively, with a methanolic running solution containing 1.0% HPMC and 0.5% PB. In the NACE-LVSEP analysis of Ru(bpy)3 and Ru(phen)3, furthermore, the values of SEF were almost 40 with almost no-loss of the resolution.