Pharmacokinetic Evaluation and Dosage Optimization of Ketoprofen in Healthy Beetal Goats

Abstract

Received: Revised: Accepted: Published online: February 19, 2019 March 04, 2019 March 12, 2019 April 06, 2019 Ketoprofen (KTP) is a frequently used non-steroidal anti-inflammatory drug (NSAID) in veterinary practice for the treatment of different inflammatory conditions. This work was carried out to evaluate the pharmacokinetic parameters of ketoprofen in healthy beetal goats. Eight goats were administered single intravenous (IV) dose of KTP (3.0mg/kg BW). Blood samples from all the goats were drawn before drug administration and then at different time intervals post administration. The KTP in plasma was estimated by using a high performance liquid Chromatography (HPLC) method. Mobile phase was mixture of phosphate buffer and acetonitrile (75:25 v/v). C18 column was used as stationary phase. The flow rate was 1.0ml/minute and temperature of column oven was adjusted to 30°C. Injection volume was 20μl. Wavelength was adjusted to 254nm. The pharmacokinetic parameters of KTP in beetal goats were calculated from plasma concentration-time data with APO MW/PHARM version 3.02 pharmacokinetic software using two compartmental model. The concentration of drug in plasma at different time intervals was calculated with regression/correlation analysis. All data are reported as mean ± SEM. The pharmacokinetic parameters determined are area under the curve (AUC) 7.711±0.60μg.h/ml, maximum concentration (Cmax) 13.64±0.98μg/ml, clearance (Cl) 0.325±0.02L/h/kg, volume of distribution (VD) 1.40±0.132L/kg, steady state volume of distribution (VDss) 0.50±0.09L/kg, half-life (t1/2) 3.10±0.37hrs, and elimination constant (Kel) 2.09±0.29L/hr. Results showed rapid elimination of ketoprofen from goat. Based on pharmacokinetics parameters, KTP at dosage of 2.47mg/kg BW is appropriate in beetal goats and may be repeated after 12 hours. ©2019 PVJ. All rights reserved