A simple tool to assess the effect of water-soluble soil pollutants on enzyme activity in human whole blood samples using WST-1 assay

IF 0.4 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Revista Internacional De Contaminacion Ambiental Pub Date : 2023-02-16 DOI:10.20937/rica.54549
Vanya Koleva, T. Koynova, Ivaila Kuleva, A. Dragoeva
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Abstract

Hazardous compounds accumulated in soil affect soil quality. Here we show that WST-1 can be used for evaluation of the effect of water soil extracts on enzyme activity in human whole-blood samples. Soil samples from the Shumen Plateau Nature Park (NP) and the City Park in Shumen (SCP) were studied. We observed significant differences between control and treated whole-blood samples. These results indicated that soil water extracts affected normal metabolic activity. In conclusion, the WST-1 assay can be used for biomonitoring soil quality. The presence of harmful water-soluble compounds in tested soils may pose a risk to ecosystems and human health.
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使用WST-1测定法评估水溶性土壤污染物对人全血样品酶活性影响的简单工具
土壤中积累的有害化合物会影响土壤质量。在这里,我们表明WST-1可以用于评估水土提取物对人类全血样品中酶活性的影响。对蜀门高原自然公园(NP)和蜀门城市公园(SCP)的土壤样品进行了研究。我们观察到对照组和治疗组全血样本之间存在显著差异。这些结果表明,土壤水分提取物影响正常代谢活性。总之,WST-1可用于土壤质量的生物监测。测试土壤中存在的有害水溶性化合物可能对生态系统和人类健康构成风险。
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CiteScore
0.90
自引率
16.70%
发文量
59
期刊介绍: En esta revista se aceptan para su publicación trabajos originales y de revisión sobre aspectos físicos y químicos de la contaminación, investigaciones sobre la distribución y los efectos biológicos y ecológicos de los contaminantes; así como sobre tecnología e implementación de nuevas técnicas para su medida y control; también son aceptados estudios sociológicos, económicos y legales acerca del tema. Se publicarán los escritos que mediante arbitraje de especialistas y a juicio del Consejo Editorial tengan el nivel y la calidad adecuados para ello y su contenido será responsabilidad única de los autores. La Revista Internacional de Contaminación Ambiental es de periodicidad trimestral y se publica los días 1 de febrero, mayo, agosto y noviembre.
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