HPLC combined with chemometrics for quality control of Huamoyan Granules or Capsules

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2024-07-01 DOI:10.1016/j.chmed.2023.03.005
Minhang Dou , Jiayi Huang , Mimi Yu , Huahua Li , Yang Song , Ziwei Peng , Shouying Du , Jie Bai
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引用次数: 0

Abstract

Objective

Huamaoyan Granules (HMYG) and Huamaoyan Capsules (HMYC) are Chinese patent medicines with different dosage forms of the same prescription. Due to the different preparation process, the chemical composition of these Chinese patent medicines varies greatly among different forms, but there were few studies on the difference comparison and quality control of them. In order to improve the effectiveness and safety in its clinical application, an idea combining high performance liquid chromatography (HPLC) and chemometrics was put forward to study the quality control of Chinese patent medicines in different dosage forms of the same prescription.

Methods

The differential markers of HMYG and HMYC were explored based on HPLC fingerprint and chemometrics including orthogonal projections to latent structures-discriminant analysis (OPLS-DA), principal component analysis (PCA), and hierarchical cluster analysis (HCA). Finally, the quantitative analysis method of related components was established by HPLC.

Results

A quality control method for HMYG and HMYC was established. Firstly, the chemical components of HMYG and HMYC were systematically analyzed by HPLC fingerprinting. Further exploration showed that there were 20 characteristic peaks and 57 common peaks. Then, the potential differential markers between HMYG and HMYC were explored by chemometrics, and the differential markers were screened after intersection with the 20 characteristic peaks. Finally, HPLC quantitative analysis methods for nine components were established, including seven differential markers (neochlorogenic acid, protocatechualdehyde, chlorogenic acid, cryptochlorogenic acid, caffeic acid, rosmarinic acid and salvianolic acid A). The results of HPLC quantitative analysis showed that the contents of eight components in HMYG and HMYC samples were significantly different. According to the above results, the differential markers between HMYG and HMYC screened based on HPLC fingerprint and chemometrics can effectively characterize the differences between the two dosage forms.

Conclusion

The present work provides a rapid and effective method for routine quality evaluation and control of HMYG and HMYC. This work also provides feasible methods for the quality evaluation and control of Chinese patent medicines with different dosage forms of the same prescription.

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高效液相色谱法联合化学计量学测定化脓炎颗粒或胶囊的质量
目的 华茂炎颗粒(HMYG)和华茂炎胶囊(HMYC)是同处方不同剂型的中成药。由于制备工艺不同,不同剂型的中成药化学成分差异较大,但有关其差异比较和质量控制的研究较少。方法基于高效液相色谱指纹图谱和化学计量学方法,包括潜结构正交投影-判别分析(OPLS-DA)、主成分分析(PCA)和层次聚类分析(HCA),探讨 HMYG 和 HMYC 的差异标记。结果 建立了 HMYG 和 HMYC 的质量控制方法。首先,通过高效液相色谱指纹图谱对 HMYG 和 HMYC 的化学成分进行了系统分析。进一步分析表明,HMYG 和 HMYC 有 20 个特征峰和 57 个常见峰。然后,利用化学计量学方法探索了 HMYG 和 HMYC 之间潜在的差异标记物,并与 20 个特征峰相交后筛选出差异标记物。最后,建立了 9 种成分的 HPLC 定量分析方法,其中包括 7 种差异标记物(新绿原酸、原儿茶醛、绿原酸、隐绿原酸、咖啡酸、迷迭香酸和丹酚酸 A)。高效液相色谱定量分析结果表明,HMYG 和 HMYC 样品中八种成分的含量存在显著差异。根据上述结果,基于高效液相色谱指纹图谱和化学计量学方法筛选出的 HMYG 和 HMYC 差异标记可有效表征两种剂型的差异。本研究为 HMYG 和 HMYC 的常规质量评价和控制提供了快速有效的方法,也为同处方不同剂型中成药的质量评价和控制提供了可行的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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文献相关原料
公司名称产品信息其他信息采购帮参考价格
上海源叶 Cryptochlorogenic acid
≥98%
¥169.00~¥17906.00
上海源叶 Neochlorogenic acid
≥98%
¥270.00~¥13068.00
上海源叶 Neoastilbin
≥98%
¥413.00~¥8340.00
来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
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