{"title":"Study of Biodeterioration Potential of Microorganisms Isolated in the Paintings Storeroom of Mouze Makhsus Museum, Golestan Palace, Tehran","authors":"N. Noohi, Moslem Papizadeh","doi":"10.1080/00393630.2022.2118269","DOIUrl":null,"url":null,"abstract":"ABSTRACT Paintings are essential components of cultural heritage all over the world. They consist of organic molecules such as oils, waxes, gums, sugars, polysaccharides, and proteins that can support the growth of various microorganisms. In this study, in order to identify the microbial community possibly involved in deterioration of paintings in the storeroom of Mouze Makhsus, culturable bacteria and fungi colonizing the paintings were isolated. The identification of isolated bacteria and fungi was performed through morphological analyses and either PCR amplification and DNA sequencing of 16srRNA gene, for bacteria, and ITS genomic region, for fungi. Based on the obtained 16S rRNA gene sequence of bacteria and ITS region sequence of fungi, a phylogenetic tree was constructed using the Neighbor-Joining method. The study also evaluated, in vitro, the cellulolytic activity of the bacterial and fungal isolates. The results highlighted the diverse array of microbial groups colonizing the sampled paintings. Culture-based analyses revealed 27 isolates of bacteria and fungi. Detected fungi fell in seven genera including Aspergillus, Penicillium, Cladosporium, Alternaria, Curvularia, Chaetomium, and Trametes. The majority of isolated bacteria belong to Bacillus genus, whereas to a lesser extent Staphylococcus, Micrococcus, Paenibacillus, Arthrobacter, Heyndrickxia, Priestia, and Rathayibacter were isolated. Qualitative and quantitative assessment of cellulolytic enzyme production showed that the fungal isolates present important cellulolytic activity, and they are potentially dangerous to the paintings.","PeriodicalId":21990,"journal":{"name":"Studies in Conservation","volume":"68 1","pages":"720 - 730"},"PeriodicalIF":0.8000,"publicationDate":"2022-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Studies in Conservation","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/00393630.2022.2118269","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"0","JCRName":"ARCHAEOLOGY","Score":null,"Total":0}
引用次数: 2
Abstract
ABSTRACT Paintings are essential components of cultural heritage all over the world. They consist of organic molecules such as oils, waxes, gums, sugars, polysaccharides, and proteins that can support the growth of various microorganisms. In this study, in order to identify the microbial community possibly involved in deterioration of paintings in the storeroom of Mouze Makhsus, culturable bacteria and fungi colonizing the paintings were isolated. The identification of isolated bacteria and fungi was performed through morphological analyses and either PCR amplification and DNA sequencing of 16srRNA gene, for bacteria, and ITS genomic region, for fungi. Based on the obtained 16S rRNA gene sequence of bacteria and ITS region sequence of fungi, a phylogenetic tree was constructed using the Neighbor-Joining method. The study also evaluated, in vitro, the cellulolytic activity of the bacterial and fungal isolates. The results highlighted the diverse array of microbial groups colonizing the sampled paintings. Culture-based analyses revealed 27 isolates of bacteria and fungi. Detected fungi fell in seven genera including Aspergillus, Penicillium, Cladosporium, Alternaria, Curvularia, Chaetomium, and Trametes. The majority of isolated bacteria belong to Bacillus genus, whereas to a lesser extent Staphylococcus, Micrococcus, Paenibacillus, Arthrobacter, Heyndrickxia, Priestia, and Rathayibacter were isolated. Qualitative and quantitative assessment of cellulolytic enzyme production showed that the fungal isolates present important cellulolytic activity, and they are potentially dangerous to the paintings.
期刊介绍：
Studies in Conservation is the premier international peer-reviewed journal for the conservation of historic and artistic works. The intended readership includes the conservation professional in the broadest sense of the term: practising conservators of all types of object, conservation, heritage and museum scientists, collection or conservation managers, teachers and students of conservation, and academic researchers in the subject areas of arts, archaeology, the built heritage, materials history, art technological research and material culture.
Studies in Conservation publishes original work on a range of subjects including, but not limited to, examination methods for works of art, new research in the analysis of artistic materials, mechanisms of deterioration, advances in conservation practice, novel methods of treatment, conservation issues in display and storage, preventive conservation, issues of collection care, conservation history and ethics, and the history of materials and technological processes. Scientific content is not necessary, and the editors encourage the submission of practical articles, review papers, position papers on best practice and the philosophy and ethics of collecting and preservation, to help maintain the traditional balance of the journal. Whatever the subject matter, accounts of routine procedures are not accepted, except where these lead to results that are sufficiently novel and/or significant to be of general interest.
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