LC-MS/MS shotgun proteomics reveals biochemical mechanisms of Paspalum fasciculatum tolerance to Pb-stress

IF 0.8 4区 生物学 Q4 PLANT SCIENCES Biologia Plantarum Pub Date : 2022-08-08 DOI:10.32615/bp.2022.016
M. Salas-Moreno, M. Castillejo, C. López-Hidalgo, J. Marrugo-Negrete, E. Rodríguez-Cavallo, D. Méndez-Cuadro, J. Jorrín-Novo
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Abstract

Paspalum fasciculatum Willd. ex Flüggé grows in mining soils which are Cd- and Pb-contaminated where it exhibits tolerance to Pb and the ability to extract Pb from these soils. To elucidate tolerance mechanisms to Pb-stress, liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to quantify changes in the accumulation of proteins in leaves. We identified 323 proteins involved in primary metabolism and response to biotic or abiotic stresses. Although proteins involved in the processes of photosynthesis and saccharide and energy metabolism presented the greatest amount of down-regulated proteins, the plant was able to maintain photosynthetic functions and obtain energy to sustain the vital balance. P. fasciculatum based their tolerance on increased antioxidant defenses, improving the protection and repair of proteins and transduction signals to coordinate physiological response to Pb-stress. Our results provide important information to understand the tolerance mechanisms in P. fasciculatum and could be important in future molecular studies on the resistance and accumulation of Pb in plants.
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LC-MS/MS霰弹枪蛋白质组学揭示雀稗耐铅胁迫的生化机制
野雀稗。ex flflgg生长在镉和铅污染的矿区土壤中,表现出对铅的耐受性和从这些土壤中提取铅的能力。为了阐明对铅胁迫的耐受机制,采用液相色谱-串联质谱(LC-MS/MS)技术定量分析了叶片中蛋白质积累的变化。我们确定了323种蛋白质参与初级代谢和对生物或非生物应激的反应。虽然参与光合作用和糖、能量代谢过程的蛋白下调量最大,但植物仍能维持光合作用功能,获取能量维持生命平衡。束状藤的耐受性是基于抗氧化防御能力的增强、蛋白质的保护和修复能力的提高以及对铅胁迫生理反应的转导信号的协调。本研究结果为了解束状藤对Pb的抗性机制提供了重要信息,并对今后植物对Pb的抗性和积累的分子研究具有重要意义。
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来源期刊
Biologia Plantarum
Biologia Plantarum 生物-植物科学
CiteScore
2.80
自引率
0.00%
发文量
28
审稿时长
3.3 months
期刊介绍: BIOLOGIA PLANTARUM is an international journal for experimental botany. It publishes original scientific papers and brief communications, reviews on specialized topics, and book reviews in plant physiology, plant biochemistry and biophysics, physiological anatomy, ecophysiology, genetics, molecular biology, cell biology, evolution, and pathophysiology. All papers should contribute substantially to the current level of plant science and combine originality with a potential general interest. The journal focuses on model and crop plants, as well as on under-investigated species.
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