Therapeutic potential and physicochemical standardisation of Lesotho propolis based on geographical location and botanical sources: a pilot study in Mohale’s Hoek district

Q2 Agricultural and Biological Sciences Transactions of The Royal Society of South Africa Pub Date : 2022-09-02 DOI:10.1080/0035919X.2022.2163000

Oriel Hlokoane, Tankiso Lechesa, Letsekha Mafereka, Mosuoenyane Moshoeshoe, Relebohile Mautsoe, Monantha Hlabi, Mpolokeng Ramats’ella, Kali Mosothoane, Ts’elleng Moleko, Motseki Mohloki, Mpho Rasenyalo

{"title":"Therapeutic potential and physicochemical standardisation of Lesotho propolis based on geographical location and botanical sources: a pilot study in Mohale’s Hoek district","authors":"Oriel Hlokoane, Tankiso Lechesa, Letsekha Mafereka, Mosuoenyane Moshoeshoe, Relebohile Mautsoe, Monantha Hlabi, Mpolokeng Ramats’ella, Kali Mosothoane, Ts’elleng Moleko, Motseki Mohloki, Mpho Rasenyalo","doi":"10.1080/0035919X.2022.2163000","DOIUrl":null,"url":null,"abstract":"We evaluated the therapeutic potential and physicochemical characteristics of propolis samples collected from three councils, namely Khoelenya (F03), Lithipeng (F04) and Thaba-mokhele (F05), in the Mohale's Hoek district, Lesotho. The aim of this study was to investigate the relationship of the therapeutic potential, physicochemical characteristics and colour variation to the geographical location and botanical sources of the collected propolis samples. The collected samples presented remarkable colour variation, ranging from green (25%) to brown (58%) to grey (17%). The highest antioxidant activity was observed in green-coloured propolis samples collected from Lithipeng F04P01 and Khoelenya F03P01, with 2,2–diphenyl–1–picryl–hydrazyl–hydrate (DPPH) half maximal inhibitory concentration (IC50) values of 0.21 and 0.23 mg/mL, respectively. The antioxidant activity (DPPH IC50) did not correlate completely with the total polyphenolic content (R 2 = 0.1733) and total flavonoid content (R 2 = 0.4836). Moreover, the highest antimicrobial activity was observed on grey propolis collected from Thaba-mokhele, F05P04 and F05P03, with minimum inhibitory concentration of 3.13 mg/mL for both samples, especially against Staphylococcus aureus. The qualitative phytochemical analysis detected the presence of polyphenols, alkaloids and flavonoids in all collected samples. Thus, our findings could lead to the formulation of a “local” Lesotho type of propolis that could be used as an official medicine. This could be a big marketing advantage for the Lesotho pharmaceutical and beekeeping industries.","PeriodicalId":23255,"journal":{"name":"Transactions of The Royal Society of South Africa","volume":"77 1","pages":"247 - 253"},"PeriodicalIF":0.0000,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transactions of The Royal Society of South Africa","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/0035919X.2022.2163000","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 0

Abstract

We evaluated the therapeutic potential and physicochemical characteristics of propolis samples collected from three councils, namely Khoelenya (F03), Lithipeng (F04) and Thaba-mokhele (F05), in the Mohale's Hoek district, Lesotho. The aim of this study was to investigate the relationship of the therapeutic potential, physicochemical characteristics and colour variation to the geographical location and botanical sources of the collected propolis samples. The collected samples presented remarkable colour variation, ranging from green (25%) to brown (58%) to grey (17%). The highest antioxidant activity was observed in green-coloured propolis samples collected from Lithipeng F04P01 and Khoelenya F03P01, with 2,2–diphenyl–1–picryl–hydrazyl–hydrate (DPPH) half maximal inhibitory concentration (IC50) values of 0.21 and 0.23 mg/mL, respectively. The antioxidant activity (DPPH IC50) did not correlate completely with the total polyphenolic content (R 2 = 0.1733) and total flavonoid content (R 2 = 0.4836). Moreover, the highest antimicrobial activity was observed on grey propolis collected from Thaba-mokhele, F05P04 and F05P03, with minimum inhibitory concentration of 3.13 mg/mL for both samples, especially against Staphylococcus aureus. The qualitative phytochemical analysis detected the presence of polyphenols, alkaloids and flavonoids in all collected samples. Thus, our findings could lead to the formulation of a “local” Lesotho type of propolis that could be used as an official medicine. This could be a big marketing advantage for the Lesotho pharmaceutical and beekeeping industries.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

基于地理位置和植物来源的莱索托蜂胶的治疗潜力和理化标准化:在Mohale的Hoek地区进行的一项试点研究

我们评估了从莱索托Mohale’s Hoek区的Khoelenya（F03）、Lithipeng（F04）和Thaba mokhele（F05）三个委员会收集的蜂胶样本的治疗潜力和理化特征。本研究的目的是研究采集的蜂胶样品的治疗潜力、理化特性和颜色变化与地理位置和植物来源的关系。采集的样本呈现出显著的颜色变化，从绿色（25%）到棕色（58%）再到灰色（17%）。在从Lithipeng F04P01和Khoelenya F03P01采集的绿色蜂胶样品中观察到最高的抗氧化活性，2,2-二苯-1-苦基-水合物（DPPH）的半最大抑制浓度（IC50）值分别为0.21和0.23 mg/mL。抗氧化活性（DPPH IC50）与总多酚含量不完全相关（R2 = 0.1733）和总黄酮含量（R2 = 0.4836）。此外，从Thaba mokhele、F05P04和F05P03采集的灰蜂胶具有最高的抗菌活性，两种样品的最低抑菌浓度均为3.13mg/mL，尤其是对金黄色葡萄球菌。定性植物化学分析在所有采集的样品中检测到多酚、生物碱和类黄酮的存在。因此，我们的研究结果可能会导致一种“当地”莱索托蜂胶的配方，可以用作官方药物。这可能是莱索托制药和养蜂业的一大营销优势。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Transactions of The Royal Society of South Africa Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

2.80

自引率

0.00%

发文量

期刊介绍： Transactions of the Royal Society of South Africa , published on behalf of the Royal Society of South Africa since 1908, comprises a rich archive of original scientific research in and beyond South Africa. Since 1878, when it was founded as Transactions of the South African Philosophical Society, the Journal’s strength has lain in its multi- and inter-disciplinary orientation, which is aimed at ‘promoting the improvement and diffusion of science in all its branches’ (original Charter). Today this includes natural, physical, medical, environmental and earth sciences as well as any other topic that may be of interest or importance to the people of Africa. Transactions publishes original research papers, review articles, special issues, feature articles, festschriften and book reviews. While coverage emphasizes southern Africa, submissions concerning the rest of the continent are encouraged.