Y. L. Xu, Yaolei Zhang, J. M. Li, Tianpeng Gao, L. N. Zhang, L. Si, Qiang Li, G. Li, Y. Yang
{"title":"Comparison of antioxidant enzyme activity and gene expression in two new spring wheat cultivars treated with salinity","authors":"Y. L. Xu, Yaolei Zhang, J. M. Li, Tianpeng Gao, L. N. Zhang, L. Si, Qiang Li, G. Li, Y. Yang","doi":"10.32615/BP.2020.171","DOIUrl":null,"url":null,"abstract":"This study aimed to analyze element content, antioxidative response, and related gene expression in two new wheat (Tritium aestivum L.) cultivars Longchun 30 and Longchun 27 when exposed to different NaCl concentrations. Low NaCl concentration (25 mM) promoted root growth and decreased malondialdehyde (MDA) content and relative conductivity (REC) in Longchun 30. Differently, higher salinity stress (100 and 200 mM NaCl) inhibited root growth and increased MDA content and REC in both cultivars. Under salt stress, the increment of Na content in the roots and leaves and the reduction of Ca content in the roots were more remarkable in Longchun 27 than in Longchun 30. In contrast, the potassium content decreased in the roots but did not significantly change in the leaves in both cultivars under salinity. When the seedlings were exposed to salinity, the increases of superoxide dismutase (SOD) and catalase (CAT) activities in Longchun 27 roots were associated with high isoenzymes abundance and high TaCu/ZnSOD, TaMnSOD and TaCAT expression. Meanwhile, total peroxidase (POD) activity induced by NaCl treatment coincided with the changes of TaPOD expression and isoenzyme abundance in both cultivars. Besides, the inhibition of activities of apoplastic antioxidant enzymes, cell wall-bound POD, diamine oxidase, and polyamine oxidase was observed in salinity-stressed roots of both cultivars. Taken together, cv. Longchun 30 might be more suitable for growing in salinity environment in comparison with Longchun 27.","PeriodicalId":8912,"journal":{"name":"Biologia Plantarum","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2021-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biologia Plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.32615/BP.2020.171","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 2
Abstract
This study aimed to analyze element content, antioxidative response, and related gene expression in two new wheat (Tritium aestivum L.) cultivars Longchun 30 and Longchun 27 when exposed to different NaCl concentrations. Low NaCl concentration (25 mM) promoted root growth and decreased malondialdehyde (MDA) content and relative conductivity (REC) in Longchun 30. Differently, higher salinity stress (100 and 200 mM NaCl) inhibited root growth and increased MDA content and REC in both cultivars. Under salt stress, the increment of Na content in the roots and leaves and the reduction of Ca content in the roots were more remarkable in Longchun 27 than in Longchun 30. In contrast, the potassium content decreased in the roots but did not significantly change in the leaves in both cultivars under salinity. When the seedlings were exposed to salinity, the increases of superoxide dismutase (SOD) and catalase (CAT) activities in Longchun 27 roots were associated with high isoenzymes abundance and high TaCu/ZnSOD, TaMnSOD and TaCAT expression. Meanwhile, total peroxidase (POD) activity induced by NaCl treatment coincided with the changes of TaPOD expression and isoenzyme abundance in both cultivars. Besides, the inhibition of activities of apoplastic antioxidant enzymes, cell wall-bound POD, diamine oxidase, and polyamine oxidase was observed in salinity-stressed roots of both cultivars. Taken together, cv. Longchun 30 might be more suitable for growing in salinity environment in comparison with Longchun 27.
研究了小麦新品种龙春30号和龙春27号在不同NaCl浓度下的元素含量、抗氧化反应及相关基因表达。低NaCl浓度(25 mM)促进了龙春30根系生长,降低了丙二醛(MDA)含量和相对电导率(REC)。不同的是,高盐胁迫(100和200 mM NaCl)抑制了两个品种的根系生长,增加了MDA含量和REC。在盐胁迫下,龙春27的根和叶中Na含量的增加和根中Ca含量的降低比龙春30更为显著。盐胁迫下,两个品种的根钾含量下降,但叶片钾含量变化不显著。幼苗受盐胁迫后,龙春27根系超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性升高,同工酶丰度高,TaCu/ZnSOD、TaMnSOD和TaCAT表达量高。同时,NaCl处理诱导的总过氧化物酶(POD)活性与TaPOD表达量和同工酶丰度的变化一致。此外,两种品种的根外胞体抗氧化酶、细胞壁结合POD、二胺氧化酶和多胺氧化酶活性均受到抑制。综上所述,cv。与龙春27相比,龙春30可能更适合在盐碱环境下生长。
期刊介绍:
BIOLOGIA PLANTARUM is an international journal for experimental botany. It publishes original scientific papers and brief communications, reviews on specialized topics, and book reviews in plant physiology, plant biochemistry and biophysics, physiological anatomy, ecophysiology, genetics, molecular biology, cell biology, evolution, and pathophysiology. All papers should contribute substantially to the current level of plant science and combine originality with a potential general interest. The journal focuses on model and crop plants, as well as on under-investigated species.