Antimicrobial, Cytotoxic, and α-Glucosidase Inhibitory Potentials Using the One Strain Many Compounds Technique for Red Sea Soft Corals Associated Fungi’ Secondary Metabolites and Chemical Composition Correlations

Pub Date : 2021-11-02 DOI:10.1080/22311866.2021.1978862

Zeinab A. El-Shahid, Faten K Abd El-Hady, W. Fayad, M. Abdel‐Aziz, Eman M. Abd EL-Azeem, Emad K Ahmed

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Abstract

Abstract Aspergillus unguis SPMD-EGY (A. unguis) and Aspergillus sp. 2C1-EGY (Aspergillus sp.) were isolated from the soft corals Sinularia and Lobophyton sp in the Hurghada coast, Red Sea, Egypt. One strain many compounds (OSMAC) was applied to trigger fungal silent biosynthetic genes. The results revealed that the A. unguis static filtrate extracts (St F) of all tested media exhibited the highest antimicrobial activity (inhibition zones 18.5-30.5 mm) against S. aureus, P. aeruginosa, and C. albicans. The A. unguis shake cell extracts of media A and C exhibited the highest α-glucosidase inhibitory activity (Sh Cell, 78 and 82 %). A high degree of acetylcholinesterase enzyme inhibition was obtained from A. unguis (Sh F, 61-66 %) shake filtrate extracts. The A. unguis extracts (A St F and D St Cell) demonstrated the highest cytotoxic activity against cancer cells, namely, HepG2, MCF-7, and HCT-116 (%I: 62-95; IC50: 7.9-90.8 µg/mL). The A. unguis extracts (A St F&D St F) exhibited high cytotoxic activities against HCT116 cancer spheroids compared with cisplatin (42.2 % and 51 %, respectively, vs 32.6 % for cisplatin). The biological activities of the extracts were due to the presence of various secondary metabolites detected by GC/MS analysis. Further pharmacological studies may lead to the isolation of new antitumor compounds besides the prospective antimicrobial and α-glucosidase inhibition capacity of the active extracts. Graphical abstract

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一株多化合物技术对红海软珊瑚伴生真菌次生代谢物的抑菌、细胞毒和α-葡萄糖苷酶抑制电位及化学成分相关性的研究

摘要:从埃及红海赫尔格达海岸的软珊瑚Sinularia和loophyton sp中分离到unguis Aspergillus SPMD-EGY和Aspergillus sp. 2C1-EGY。其中一株多化合物(OSMAC)用于触发真菌沉默的生物合成基因。结果表明，所有培养基中金鸡假单胞菌静态滤液提取物(St F)对金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌的抑菌活性最高(抑制区为18.5 ~ 30.5 mm)。培养基A和C的黄颡鱼摇细胞提取物对α-葡萄糖苷酶的抑制活性最高(Sh cell, 78%和82%)。钉螺(Sh F, 61- 66%)摇滤液提取物对乙酰胆碱酯酶有较好的抑制作用。A. unguis提取物(A . St . F和D . St . Cell)对癌细胞HepG2、MCF-7和HCT-116的细胞毒活性最高(%I: 62-95;IC50: 7.9-90.8µg/mL)。与顺铂相比，刺荆芥提取物(A St F&D St F)对HCT116癌球体具有较高的细胞毒活性(分别为42.2%和51%，而顺铂为32.6%)。通过GC/MS分析，发现提取物中存在多种次生代谢物，具有一定的生物活性。进一步的药理研究可能会导致新的抗肿瘤化合物的分离，除了预期的抗菌和α-葡萄糖苷酶抑制能力的活性提取物。图形抽象

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