Genetic variation of GLI-B1 locus in ukrainian bread wheat varieties and lines

Q4 Biochemistry, Genetics and Molecular Biology Biopolymers and Cell Pub Date : 2021-10-30 DOI:10.7124/bc.000a63
Yulia Popovych, O. M. Blagodarova, S. Chebotar
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Abstract

Aim. To investigate polymorphism of Gli-B1 locus in modern Ukrainian bread wheat cultivars, to analyze the distribution of alleles and to compare the received data with the “core-collection of wheat cultivars” presented by Dr. E. Metakovsky. Methods. Eighty one bread wheat cultivars and lines from different plant breeding institutions and stations of Ukraine were tested using allele-specific primers to Gli-B1 locus developed by Zhang et al. [2003]. PCR products were fractionated in polyacrylamide gel (PAG) and then were stained by silver nitrate. Allelic variants of gliadins were analyzed by electrophoresis in acid polyacrylamide gel (APAGE). Results. Nine allelic variants of gliadins were revealed by APAGE and six alleles of Gli-B1 locus were detected by PCR-analysis. In 52 % of modern Ukrainian bread wheat cultivars we revealed Gli-B1b allelic variant, according to PCR - Gli-B1.1 allele with a 369 bp amplification fragment. In the genotypes of Ukrainian wheat cultivars, the 1RS.1BL translocation, which carries resistance genes, is frequent, as was detected by the absence of any amplification fragments with Gli-B1 primers. The correspondence between allelic variants of gliadins and alleles of Gli-B1 locus is discussed. Conclusions. DNA polymorphism of Gli-B1 locus examined in our research coincides with the diversity of allelic variants of gliadins, which were detected by APAGE method for Ukrainian bread wheat cultivar. However, PCR-analysis with applied primers carried out in this study does not distinguish the alleles that correspond to the Gli-B1c, Gli-B1g and Gli-B1e allelic variants of gliadins. The most common allele (52 %) for the in-vestigated Ukrainian wheat varieties is Gli-B1.1 allele, which was characterized by the amplification fragment of 369 bp, and the presence of 1RS.1BL translocation, which corresponds to Gli-B1b and Gli-B1l allelic variants of gliadins obtained by APAGE, respectively.
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乌克兰面包小麦品种和品系GLI-B1基因座的遗传变异
目标研究现代乌克兰面包小麦品种中Gli-B1基因座的多态性,分析等位基因的分布,并将收到的数据与E.Metakovsky博士提出的“小麦品种核心集合”进行比较。方法。使用Zhang等人开发的Gli-B1基因座等位基因特异性引物对来自乌克兰不同植物育种机构和站点的81个面包小麦品种和品系进行了测试。[2003]。PCR产物在聚丙烯酰胺凝胶(PAG)中分级,然后用硝酸银染色。用酸性聚丙烯酰胺凝胶电泳分析了醇溶蛋白的等位基因变异。后果APAGE检测到9个醇溶蛋白等位基因变异,PCR检测到6个Gli-B1等位基因。在52%的现代乌克兰面包小麦品种中,根据PCR-Gli-B1.1等位基因和369bp的扩增片段,我们发现了Gli-B1b等位基因变体。在乌克兰小麦品种的基因型中,携带抗性基因的1RS.1BL易位是频繁的,正如用Gli-B1引物没有任何扩增片段所检测到的那样。讨论了醇溶蛋白等位基因变异与Gli-B1基因座等位基因的对应关系。结论。本研究检测的Gli-B1基因座的DNA多态性与用APAGE方法检测的乌克兰面包小麦品种醇溶蛋白等位基因变异的多样性一致。然而,本研究中使用应用引物进行的PCR分析并不能区分对应于醇溶蛋白的Gli-B1c、Gli-B1g和Gli-B1e等位基因变体的等位基因。在研究的乌克兰小麦品种中,最常见的等位基因(52%)是Gli-B1.1等位基因,其特征是369bp的扩增片段和1RS.1BL易位的存在,其分别对应于APAGE获得的醇溶蛋白的Gli-B1b和Gli-B1l等位基因变体。
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来源期刊
Biopolymers and Cell
Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.10
自引率
0.00%
发文量
9
期刊介绍: “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.
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