Chlorogenic Acid Inhibits Proliferation, Migration and Invasion of Pancreatic Cancer Cells via AKT/GSK-3β/β-catenin Signaling Pathway.

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-01-01 DOI:10.2174/1574892818666230327134746

Xiaoliang Chen, Binyu Liu, Jiale Tong, Jianing Bo, Miao Feng, Lili Yin, Xiukun Lin

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Abstract

Background: Chlorogenic acid (CA, United States Patent No. 10772340), a natural biologically active food ingredient, displays potent antitumor activity against a variety of cancer cells. However, the mechanism underlying its anticancer effect is not well elucidated.

Objective: In the present study, we hope to dissect the mechanism underlying the anticancer effects of CA in pancreatic cancer cells.

Methods: The cytotoxicity of CA in pancreatic cancer cells was determined by MTT assay. Flow cytometry was performed to evaluate the cells apoptosis, while a clonogenic assay was carried out to check the colony formation of cancer cells. Transwell assay was performed to assess the cells migration and invasion. The protein expression of AKT/GSK-3β/β-catenin signaling pathway was detected by Western Blot.

Results: Our data indicated that CA inhibited the proliferation of PANC-28 and PANC-1 cells in a dose and time-dependent manner. CA was able to inhibit colony formation, migration, and invasion ability and trigger apoptosis in PANC-28 and PANC-1 cells. Further study showed that CA down-regulated the expression of AKT, p-AKT(Thr308), p-GSK-3β(Ser9), β-catenin, N-cadherin, and vimentin while enhancing the expression of cleaved-caspase 3 and cleaved-caspase 7 in PANC-28 and PANC-1 cells.

Conclusion: Our study provides significant evidence that CA is able to inhibit the growth of pancreatic cancer via the AKT/GSK-3β/β-catenin signaling pathway.

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绿原酸通过AKT/GSK-3β/β-catenin信号通路抑制胰腺癌症细胞增殖、迁移和侵袭。

绿原酸（CA，美国专利号10772340）是一种天然生物活性食品成分，对多种癌症细胞显示出强大的抗肿瘤活性。然而，其抗癌作用的机制尚不清楚。在本研究中，我们希望剖析CA在胰腺癌症细胞中抗癌作用的机制。用MTT法测定CA对癌症细胞的细胞毒性。流式细胞仪检测细胞凋亡，克隆形成检测癌症细胞集落形成。进行Transwell测定以评估细胞迁移和侵袭。Western Blotting法检测了AKT/GSK-3β/β-catenin信号通路的蛋白表达。我们的数据表明，CA以剂量和时间依赖的方式抑制PANC-28和PANC-1细胞的增殖。CA能够抑制PANC-28和PANC-1细胞的集落形成、迁移和侵袭能力，并引发细胞凋亡。进一步的研究表明，CA下调了PANC-28和PANC-1细胞中AKT、p-AKT（Thr308）、p-GSK-3β（Ser9）、β-连环蛋白、N-钙粘蛋白和波形蛋白的表达，同时增强了切割的胱天蛋白酶7的表达。我们的研究提供了重要的证据，表明CA能够通过AKT/GSK-3β/β-catenin信号通路抑制癌症的生长。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.