Intraspecific heterogeneity, multiple allelism and morphological divergence between morphotypes of Thalassiosira allenii (Bacillariophyta) from the Sea of Japan

IF 1.5 4区 生物学 Q3 MARINE & FRESHWATER BIOLOGY Phycologia Pub Date : 2023-07-04 DOI:10.1080/00318884.2023.2231783
K. Efimova, M. A. Shulgina, O. Shevchenko
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Abstract

ABSTRACT Thalassiosira allenii is reported for the first time from marine phytoplankton of the Russian far East. Five strains representing three morphological variants of T. allenii, from three locations in the Sea of Japan, were investigated. The D8–D10 domain of LSU rDNA and rbcL of T. allenii were sequenced for the first time. Three morphological forms of T. allenii showed completely identical rbcL sequences and distinctly different nuclear rDNA sequences. The genetic differences between morphotypes were determined by the number of allelic variants of LSU rDNA, one of which was common to all studied strains. Two different types of the D1–D3 domain and three different types of the D8–D10 divergent domain were detected in the cultured strains of T. allenii. The three morphotypes are described in detail and compared. One allele of D1–D3 and one allele of D8–D10 were identified in the strains of T. allenii from two stations in Ussuri Bay. Two and three alleles of the D8–D10 domain were identified in the strains with an ambiguous morphotype and in T. allenii var. striata, respectively, from one station in Amur Bay. Subdivision of T. allenii into morphotypes based on morphology matched that based on molecular-genetic analyses using D8–D10 LSU rDNA. Caution is recommended in using LSU rDNA sequences and rbcL divergence as the principal criterion for delimiting and metabarcoding T. allenii varieties/morphotypes. The lack of genetic differentiation in rbcL, and the presence of clear intra-specific genetic differentiation among morphotypes in two regions of LSU rDNA suggest a link between morphological differences and the nuclear genome. The D8–D10 domain of LSU rDNA appears more suitable for discriminating morphotypes within T. allenii. The rbcL is an effective marker for recognizing T. allenii at species level, but not at infraspecific level.
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日本海蓝藻(硅藻门)种内异质性、多等位基因及形态差异
摘要首次报道了俄罗斯远东地区海洋浮游植物中allenii thalassisira。对来自日本海3个地点的5株代表3种形态变异的allenii T.进行了调查。首次测定了褐霉LSU rDNA D8-D10结构域和rbcL序列。三种形态的黄颡鱼rbcL序列完全相同,细胞核rDNA序列差异明显。形态型之间的遗传差异由LSU rDNA等位基因变异的数量决定,其中一个等位基因变异对所有研究菌株都是共同的。在培养菌株中检测到2种不同类型的D1-D3结构域和3种不同类型的D8-D10分化结构域。对三种形态进行了详细的描述和比较。在乌苏里湾2个站点的褐僵菌株中鉴定出1个D1-D3等位基因和1个D8-D10等位基因。在阿穆尔湾一个站点的形态不明确菌株和纹状体黄颡鱼变种中分别鉴定出2个和3个D8-D10结构域等位基因。利用D8-D10 LSU rDNA进行分子遗传分析,结果与形态学分析结果相吻合。建议谨慎使用LSU rDNA序列和rbcL差异作为划分和元条形码的主要标准。rbcL缺乏遗传分化,LSU rDNA两个区域形态型之间存在明确的种内遗传分化,这表明形态差异与核基因组之间存在联系。LSU rDNA的D8-D10结构域似乎更适合于鉴别黄褐稻的形态类型。rbcL是一种在种水平上识别黄颡鱼的有效标记,但在种下水平上识别不了。
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来源期刊
Phycologia
Phycologia 生物-海洋与淡水生物学
CiteScore
3.10
自引率
18.80%
发文量
54
审稿时长
3 months
期刊介绍: Phycologia is published bimonthly by the International Phycological Society and serves as a publishing medium for information about any aspect of phycology. Membership in the Society is not necessary for publication. Submitted manuscripts cannot be previously published or submitted elsewhere. Copyright ownership of all accepted papers is held by the International Phycological Society.
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