{"title":"Detection to trace aluminum ion of pharmaceutical wastewater using synthesis of Schiff-based chemosensor","authors":"Mengistu Jemberu Dagnaw, Mahesh Gopal","doi":"10.34172/ehem.2021.35","DOIUrl":null,"url":null,"abstract":"Background: The aim of this research was to develop a fluorogenic sensor for Al3+ions, which have been identified as a possible food and drinking water pollutant by the WHO and considered to be harmful to human health. Methods: The sensing mechanism was based on excited-state intramolecular proton transfer, with the intramolecular rotation restriction occurring after binding with the analyte. The probe attaches Al3+selectively and emits strong emission in 4:1 H2 O/MeOH (v/v) solution while irradiated at 400 nm in the presence of a wide number of cations, acting as a \"turn-on\" fluorescence chemosensor. The range of detection for Al3+is 3.3 nM (3 method), which is more than 200 times more responsive than the WHO suggested limit of 7.4 mM (3σ method). Mass spectra, job plot, and Benesi-Hildebrand plot were used to determine the formation of the 1:1 metal-to-ligand complex. Results: Aluminum (Al) ion content in effluent obtained from the pharmaceutical sector is 0.381 mM, which is a trace amount. A separate in vitro experiment indicates that the probe can precisely perceive Al3+ions in a cell line. The sensor-based method is developed to detect 3.3 nM of Al3+ions, which is significantly less than the WHO max. Conclusion: The probe to detect Al3+ions in live cells. HL becomes a flexible sensor for recognizing intracellular Al3+in human liver cancer cell line Hep G2 and human lung fibroblast cell lines by fluorescence cell imaging procedures, and the probe’s non-toxicity has been proven by MTT tests up to 100M.","PeriodicalId":51877,"journal":{"name":"Environmental Health Engineering and Management Journal","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2021-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Health Engineering and Management Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34172/ehem.2021.35","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 1
Abstract
Background: The aim of this research was to develop a fluorogenic sensor for Al3+ions, which have been identified as a possible food and drinking water pollutant by the WHO and considered to be harmful to human health. Methods: The sensing mechanism was based on excited-state intramolecular proton transfer, with the intramolecular rotation restriction occurring after binding with the analyte. The probe attaches Al3+selectively and emits strong emission in 4:1 H2 O/MeOH (v/v) solution while irradiated at 400 nm in the presence of a wide number of cations, acting as a "turn-on" fluorescence chemosensor. The range of detection for Al3+is 3.3 nM (3 method), which is more than 200 times more responsive than the WHO suggested limit of 7.4 mM (3σ method). Mass spectra, job plot, and Benesi-Hildebrand plot were used to determine the formation of the 1:1 metal-to-ligand complex. Results: Aluminum (Al) ion content in effluent obtained from the pharmaceutical sector is 0.381 mM, which is a trace amount. A separate in vitro experiment indicates that the probe can precisely perceive Al3+ions in a cell line. The sensor-based method is developed to detect 3.3 nM of Al3+ions, which is significantly less than the WHO max. Conclusion: The probe to detect Al3+ions in live cells. HL becomes a flexible sensor for recognizing intracellular Al3+in human liver cancer cell line Hep G2 and human lung fibroblast cell lines by fluorescence cell imaging procedures, and the probe’s non-toxicity has been proven by MTT tests up to 100M.