Hydrophilic molecularly imprinted chitosan microspheres based on deep eutectic solvents for micro-solid phase extraction of catechin

IF 1 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of Liquid Chromatography & Related Technologies Pub Date : 2023-03-16 DOI:10.1080/10826076.2023.2208201

Mengdi Yuan, Chenglin Zhong, K. Row, Guizhen Li

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Abstract

Abstract Hydrophilic molecularly imprinted chitosan microspheres (HMICS) were synthesized using deep eutectic solvents (DESs)-(3-aminopropyl) triethoxysilane (APTES) as co-functional monomer for the extraction of catechin from green tea with micro-solid phase extraction (micro-SPE). Various factors were studied to optimize the extraction condition during extraction process. The actual extraction amount under the optimal conditions (amount of adsorbent (20 mg), type and volume of washing (acetone, 1.2 mL) and eluting solvent (acetone/acetic acid, 1.0 mL), extraction time (30 min), extraction temperature (40 °C), and sample pH (4)) were obtained at 97.06 mg·g−1. The catechin recovery ranged with ranged with limit of detection (LOD) within 0.09–0.24 µg·mL−1, and limit of quantification (LOQ) within 0.18–0.28 µg·mL−1. From the experimental results in this system, the HMICS was proved to have higher extraction capacity, improving as a promising adsorbent for the extraction of catechin. GRAPHICAL ABSTRACT

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基于深共晶溶剂的亲水分子印迹壳聚糖微球微固相萃取儿茶素

摘要以深度共晶溶剂（DESs）-（3-氨基丙基）三乙氧基硅烷（APTES）为共官能单体，采用微固相萃取法（micro-SPE）从绿茶中提取儿茶素，合成了亲水性分子印迹壳聚糖微球（HMICS）。对提取过程中的各种因素进行了研究，优化了提取条件。最佳条件下的实际提取量（吸附剂（20 mg），洗涤的类型和体积（丙酮，1.2 mL）和洗脱溶剂（丙酮/乙酸，1.0 mL），提取时间（30 min），提取温度（40 °C），样品pH（4））在97.06时获得儿茶素的回收率在0.09–0.24之间，检出限（LOD）在0.09至0.24之间 µg·mL−1，定量限（LOQ）在0.18–0.28之间 µg·mL−1。从该体系的实验结果来看，HMICS具有更高的萃取能力，是一种很有前途的儿茶素萃取吸附剂。图形摘要

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来源期刊

Journal of Liquid Chromatography & Related Technologies 化学-分析化学

CiteScore

2.80

自引率

0.00%

发文量

审稿时长

4.9 months

期刊介绍： The Journal of Liquid Chromatography & Related Technologies is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with analytical, preparative and process scale liquid chromatography and all of its related technologies, including TLC, capillary electrophoresis, capillary electrochromatography, supercritical fluid chromatography and extraction, field-flow technologies, affinity, and much more. New separation methodologies are added when they are developed. Papers dealing with research and development results, as well as critical reviews of important technologies, are published in the Journal.